Ig Kappa antibody

The Ig Kappa Light Chain is a crucial component of the immunoglobulin (Ig) molecule, which plays a significant role in the immune response. The Mouse Anti-Human Ig Kappa Light Chain antibody is a monoclonal antibody specifically designed to target and bind to the kappa light chains of human immunoglobulins. This antibody is widely used in various research and diagnostic applications, including immunohistochemistry, flow cytometry, and enzyme-linked immunosorbent assays (ELISA).

Immunoglobulins, also known as antibodies, are Y-shaped molecules composed of two heavy chains and two light chains. The light chains can be of two types: kappa (κ) or lambda (λ). Each immunoglobulin molecule contains either two kappa or two lambda light chains, but not both. The kappa light chain is encoded by the IGKC gene and is involved in the antigen-binding function of the antibody.

The Mouse Anti-Human Ig Kappa Light Chain antibody is produced by immunizing mice with human kappa light chain proteins. The spleen cells from these immunized mice are then fused with myeloma cells to create hybridoma cells. These hybridoma cells are capable of producing large quantities of monoclonal antibodies specific to the human kappa light chain. The antibodies are then purified from the hybridoma culture supernatant using protein A or G affinity chromatography.

1. Immunohistochemistry (IHC): The Mouse Anti-Human Ig Kappa Light Chain antibody is used to detect the presence of kappa light chains in tissue sections. This is particularly useful in the diagnosis of certain types of cancers, such as multiple myeloma and B-cell lymphomas, where the overexpression of kappa light chains can be indicative of malignancy.
2. Flow Cytometry: This antibody is used to analyze the expression of kappa light chains on the surface of cells. It helps in the identification and characterization of B-cell populations in various samples, including blood, bone marrow, and lymphoid tissues.
3. ELISA: The Mouse Anti-Human Ig Kappa Light Chain antibody is used as a capture or detection antibody in ELISA assays to quantify the levels of kappa light chains in biological samples. This is useful in monitoring the progression of diseases such as multiple myeloma and in assessing the efficacy of therapeutic interventions.

The Mouse Anti-Human Ig Kappa Light Chain antibody is highly specific for human kappa light chains and does not cross-react with lambda light chains or other species’ immunoglobulins. This specificity is crucial for accurate detection and quantification of kappa light chains in various applications.