Ig Lambda antibody

The immunoglobulin (Ig) light chain is a crucial component of antibodies, which are produced by B-cells. In humans, light chains can be classified into two types: kappa (κ) and lambda (λ). Each B-cell produces antibodies with either kappa or lambda light chains, but not both. The Ig lambda light chain plays a significant role in the immune response by binding to antigens and facilitating their neutralization.

The Ig lambda light chain is the smaller subunit of an antibody and is composed of a variable region and a constant region. The variable region is responsible for antigen binding, while the constant region interacts with other components of the immune system. The lambda light chain pairs with the heavy chain of the antibody to form a complete immunoglobulin molecule.

Mouse anti-human Ig lambda light chain antibodies are monoclonal antibodies produced by immunizing mice with human Ig lambda light chains. These antibodies are highly specific and can detect human Ig lambda light chains in various assays, including Western blot, ELISA, and flow cytometry .

1. Western Blot: Mouse anti-human Ig lambda light chain antibodies are used to detect the presence of lambda light chains in protein samples. They bind specifically to the lambda light chain, allowing for the visualization of these proteins on a membrane .
2. ELISA: These antibodies can be used as capture or detection antibodies in enzyme-linked immunosorbent assays (ELISA). They help quantify the amount of lambda light chain present in a sample .
3. Flow Cytometry: In flow cytometry, mouse anti-human Ig lambda light chain antibodies are used to stain cells, enabling the identification and quantification of B-cells expressing lambda light chains .