HPSE Human
Heparanase-1 Human Recombinant
Cat. No.
BT25269
Source
Escherichia Coli.
Synonyms
Heparanase, HPA, HSE1, HPA1, HPR1, HPSE1, heparanase-1, EC 3.2.1.166, Endo-glucoronidase, HEP, Heparanase-1, Hpa1.
Appearance
Sterile Filtered clear solution.
Purity
Greater
than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
HPSE Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 531 amino acids (36-543 a.a) and having a molecular mass of 60kDa.
HPSE is fused to a 23 amino acid His-tag at N-terminus.
HPSE is fused to a 23 amino acid His-tag at N-terminus.
Product Specs
Introduction
HPSE, also known as Heparanase-1, is an enzyme that breaks down heparan sulfate proteoglycans. This process allows cells to move through the extracellular matrix, which is like a scaffold surrounding cells. Heparan sulfate proteoglycans are key components of this matrix, particularly the basement membrane. Breaking them down also releases molecules that can have different effects on cells. HPSE is also important for the overall breakdown of proteins within lysosomes, which are like recycling centers within cells.
Description
This recombinant HPSE protein is produced in E. coli bacteria and is a single chain of 531 amino acids (specifically amino acids 36 to 543), with a molecular weight of 60kDa. It is not glycosylated, meaning it does not have sugars attached. The protein has a 23 amino acid His-tag attached to its N-terminus to aid in purification.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The HPSE protein is supplied in a solution with a concentration of 1mg/ml. The solution also contains 20mM Tris-HCl buffer at pH 8.0, 10% glycerol to prevent freezing, and 0.4M Urea to help keep the protein stable.
Stability
For short-term use (2-4 weeks), store the protein at 4°C. For longer storage, freeze the protein at -20°C. To preserve protein quality during long-term storage, it's recommended to add a carrier protein like HSA or BSA (0.1%). Repeated freezing and thawing of the protein should be avoided.
Purity
The purity of the HPSE protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Heparanase, HPA, HSE1, HPA1, HPR1, HPSE1, heparanase-1, EC 3.2.1.166, Endo-glucoronidase, HEP, Heparanase-1, Hpa1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSQDVVDLD FFTQEPLHLV SPSFLSVTID ANLATDPRFL ILLGSPKLRT LARGLSPAYL RFGGTKTDFL IFDPKKESTF EERSYWQSQV NQDICKYGSI PPDVEEKLRL EWPYQEQLLL REHYQKKFKN STYSRSSVDV LYTFANCSGL DLIFGLNALL RTADLQWNSS NAQLLLDYCS SKGYNISWEL GNEPNSFLKK ADIFINGSQL GEDFIQLHKL LRKSTFKNAK LYGPDVGQPR RKTAKMLKSF LKAGGEVIDS VTWHHYYLNG RTATKEDFLN PDVLDIFISS VQKVFQVVES TRPGKKVWLG ETSSAYGGGA PLLSDTFAAG FMWLDKLGLS ARMGIEVVMR QVFFGAGNYH LVDENFDPLP DYWLSLLFKK LVGTKVLMAS VQGSKRRKLR VYLHCTNTDN PRYKEGDLTL YAINLHNVTK YLRLPYPFSN KQVDKYLLRP LGPHGLLSKS VQLNGLTLKM VDDQTLPPLM EKPLRPGSSL GLPAFSYSFF VIRNAKVAAC I.
Product Science Overview
Structure and Function
Heparanase-1 is an endo-β-D-glucuronidase, which means it specifically cleaves heparan sulfate chains into smaller fragments. The enzyme is initially synthesized as a latent 65 kDa pro-heparanase, which undergoes further processing to become active. The mature, active form of heparanase-1 is a heterodimer consisting of an 8 kDa subunit and a 50 kDa subunit .
Biological Roles
- Tumor Growth and Metastasis: Heparanase-1 is highly expressed in various malignancies. Its high expression is associated with larger tumor sizes, increased metastases, and poor prognosis. By degrading heparan sulfate in the ECM, heparanase-1 facilitates tumor cell invasion and migration .
- Angiogenesis: The enzyme promotes the release of heparan sulfate-bound growth factors, such as fibroblast growth factors (FGFs) and vascular endothelial growth factors (VEGFs), which are crucial for new blood vessel formation .
- Inflammation: Heparanase-1 is involved in the release of cytokines and chemokines, contributing to inflammatory responses .
Recombinant Heparanase-1
Clinical Implications
Given its significant role in cancer progression and other pathological conditions, heparanase-1 has been a target for drug development. However, developing effective anti-heparanase therapies has been challenging due to the enzyme’s multifunctional nature and the complexity of its interactions within the body .
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