Heparanase 1 (HPA1), Polyclonal

Heparanase is produced as a latent 65 kDa precursor and is proteolytically processed into its active form, which is a heterodimer composed of a 50 kDa subunit harboring the active site and an 8 kDa subunit . Upon degradation of heparan sulfate, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis .

Heparanase is highly expressed in myeloid leukocytes, such as neutrophils, in platelets, and in the human placenta . It has been found to be upregulated in various types of primary tumors, correlating with increased tumor invasiveness and vascularity, and often with poor prognosis . This makes heparanase a significant target for cancer research and potential therapeutic interventions.

The polyclonal rabbit anti-human heparanase 1 antibody is a protein G affinity-purified polyclonal antibody raised against the 50 kDa-8 kDa heparanase heterodimer . This antibody is suitable for applications such as Western blotting and immunohistochemistry . In Western blot analysis, the antibody reacts with the 65 kDa precursor as well as the 50 kDa and 8 kDa subunits of human or mouse heparanase . In immunohistochemistry, it interacts with heparanase in paraffin sections and blood smears .

The recommended dilution range for Western blot analysis is 1:2000, and for immunohistochemistry, it is 1:100 . The antibody should be stored at 4°C for short-term use and in working aliquots at -20°C for extended storage, avoiding repeated freeze-thaw cycles .

1. Abcam - Anti-Heparanase 1 antibody (ab85543)
2. ProSpec - Heparanase 1 Antibody Polyclonal Rabbit Anti-Human
3. Fisher Scientific - Heparanase-1, Rabbit anti-Human, Mouse, Polyclonal