Heparanase 1 Clone HP3/17
Mab HP3/17 is a Protein G affinity purified monoclonal antibody raised against a polypeptide from the 50 kDa subunit of Heparanase.
>98% on SDS-PAGE when loaded 50 μg/lane.
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Description
Product Specs
Mab HP3/17 is a Protein G affinity purified monoclonal antibody raised against a polypeptide from the 50 kDa subunit of Heparanase.
HP3/17 reacts with the 50 kDa subunit and with the 65 kDa precursor of human or mouse Heparanase by Western blotting and immunohistochemistry.
Recommended dilution range for Western blot analysis: 1:4000.
Recommended dilution range for immunohistochemistry: 1:40.
Mouse IgG2Bκ
Product Science Overview
Heparanase 1 (HPA1) is an enzyme that plays a crucial role in the degradation of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. This enzyme is an endo-β-D-glucuronidase, which specifically cleaves the heparan sulfate side chains of HSPGs . The monoclonal anti-human antibody against Heparanase 1 is a significant tool in biomedical research, particularly in the study of cancer metastasis and inflammation.
Heparanase 1 is a glycosylated protein that exists in two forms: a latent 65 kDa precursor and an active 50 kDa form . The enzyme is synthesized as an inactive precursor and undergoes proteolytic cleavage to become active. The active form of Heparanase 1 degrades heparan sulfate chains, which are key components of the extracellular matrix and basement membranes .
Heparanase 1 is involved in various physiological and pathological processes. Its primary function is to remodel the extracellular matrix, facilitating cell migration and tissue remodeling. This activity is particularly important in processes such as wound healing, angiogenesis, and embryonic development . However, Heparanase 1 is also implicated in pathological conditions, including cancer metastasis and inflammation. By degrading the extracellular matrix, Heparanase 1 enables tumor cells to invade surrounding tissues and spread to distant sites .
The monoclonal anti-human Heparanase 1 antibody is a laboratory-produced molecule designed to bind specifically to Heparanase 1. This antibody is typically produced using hybridoma technology, where a single clone of B cells is fused with myeloma cells to produce a continuous cell line that secretes the desired antibody . The monoclonal antibody against Heparanase 1 is used in various research applications, including Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA) .
The monoclonal anti-human Heparanase 1 antibody is a valuable tool in biomedical research. It is used to study the expression and activity of Heparanase 1 in different tissues and under various physiological and pathological conditions. For instance, researchers use this antibody to investigate the role of Heparanase 1 in cancer progression, where its expression is often upregulated . Additionally, the antibody is employed in studies exploring the involvement of Heparanase 1 in inflammatory diseases and tissue remodeling .
