HPSE Active

Recombinant Human Heparanase-1 is expressed in human HEK 293 cells as an active heterodimer glycoprotein containing a 50 kDa subunit and an 8 kDa subunit . This expression system allows human-like glycosylation and folding, which often supports higher specific activity of the protein . The protein is produced without artificial tags, ensuring its functionality and stability.

Heparanase-1 is the only heparan sulfate-degrading endoglycosidase. It cleaves the heparan sulfate side chains of heparan sulfate proteoglycans into fragments of 10–20 sugar units . This enzyme is initially translated as a preproenzyme containing a signal peptide. The cleavage of the signal sequence yields a latent 65 kDa pro-heparanase, which must undergo further processing to become active. The mature active heparanase is a heterodimer consisting of an N-terminal 8 kDa subunit and a C-terminal 50 kDa subunit .

Heparanase-1 plays a crucial role in the regulation of multiple biological processes, including tumor growth, metastasis, angiogenesis, and inflammation . It is involved in extracellular matrix (ECM) remodeling and the release of heparan sulfate-linked biological molecules, such as cytokines and growth factors . The enzyme’s activity is measured by its ability to cleave heparan sulfate from substrates like Syndecan-4 .

Due to its involvement in critical biological processes, recombinant human heparanase-1 is extensively used in research to study cancer progression, tissue regeneration, and inflammatory diseases . Its ability to degrade heparan sulfate makes it a valuable tool for investigating the ECM’s role in various physiological and pathological conditions.

The recombinant human heparanase-1 is produced with high purity, typically greater than 95%, as determined by SDS-PAGE . It is supplied as a lyophilized powder or in a solution, depending on the manufacturer. The endotoxin level is kept below 1.0 EU per 1 μg of protein to ensure its suitability for sensitive biological applications .

For optimal stability, recombinant human heparanase-1 should be stored at -20°C to -70°C. It is essential to avoid repeated freeze-thaw cycles to maintain its activity . The protein remains stable for several months under these conditions, making it suitable for long-term research projects.