HOMER1 Human

Homer Homolog-1 Human Recombinant
Cat. No.
BT22314
Source
Escherichia Coli.
Synonyms
HOMER, SYN47, Ves-1, HOMER1A, HOMER1B, HOMER1C, HOMER1, Homer protein homolog 1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Human HOMER1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 391 amino acids (1-354 a.a) and having a molecular mass of 44.5 kDa. HOMER1 is fused to a 37 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
HOMER1 plays a role in determining how susceptible cells are to apoptosis induced by TRAIL. It belongs to the Homer protein family, which regulates signal transduction and trafficking of glutamate receptors. Additionally, these proteins maintain and regulate extracellular glutamate levels in corticolimbic brain regions. HOMER1 is primarily found at excitatory synapses, where it binds to group 1 metabotropic glutamate receptors (mGluRs).
Description
Recombinant Human HOMER1, expressed in E. coli, is a single polypeptide chain without glycosylation. It comprises 391 amino acids (specifically, amino acids 1 to 354) and has a molecular weight of 44.5 kDa. This protein includes a 37 amino acid His Tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The HOMER1 protein solution has a concentration of 1mg/ml. It is supplied in a buffer containing 20mM Tris-HCl (pH 8), 1mM DTT, 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of this protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
HOMER, SYN47, Ves-1, HOMER1A, HOMER1B, HOMER1C, HOMER1, Homer protein homolog 1.
Source
Escherichia Coli.
Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMGE QPIFSTRAHV FQIDPNTKKN WVPTSKHAVT VSYFYDSTRN VYRIISLDGS KAIINSTITPNMTFTKTSQK FGQWADSRAN TVYGLGFSSE HHLSKFAEKF QEFKEAARLA KEKSQEKMEL TSTPSQESAG GDLQSPLTPE SINGTDDERT PDVTQNSEPR AEPTQNALPF SHSSAISKHW EAELATLKGN NAKLTAALLE STANVKQWKQ QLAAYQEEAE RLHKRVTELE CVSSQANAVH THKTELNQTI QELEETLKLK EEEIERLKQE IDNARELQEQ RDSLTQKLQE VEIRNKDLEG QLSDLEQRLE KSQNEQEAFR NNLKTLLEIL DGKIFELTEL RDNLAKLLEC S.

Product Science Overview

Structure and Isoforms

Homer1 protein has a distinct structure with an N-terminal EVH1 domain involved in protein interaction and a C-terminal coiled-coil domain responsible for self-association . There are two major splice variants of Homer1:

  • Short-form (Homer1a)
  • Long-form (Homer1b and Homer1c)

These isoforms differ in their functions and interactions within the cell.

Function and Significance

Homer1 is enriched at excitatory synapses and binds to group 1 metabotropic glutamate receptors (mGluRs) . It is involved in several critical biological processes, including:

  • Regulation of signal transduction through glutamate receptors
  • Maintenance and regulation of extracellular glutamate levels in corticolimbic brain regions
  • Positive regulation of signal transduction and calcium ion transport
  • Regulation of synaptic transmission and dendritic spine maintenance
Expression and Localization

Homer1 is predominantly expressed in the brain, particularly in regions such as the Brodmann area 23, middle temporal gyrus, and orbitofrontal cortex . It is also found in various other tissues, including skeletal muscle and endothelial cells .

Recombinant Homer1

Recombinant Homer1 protein is produced using E. coli expression systems and is often tagged with a His-tag for purification purposes . This recombinant protein is used in research to study its interactions and functions in various biological processes.

Applications in Research

The recombinant Homer1 protein is valuable in research for:

  • Studying protein-protein interactions at synapses
  • Investigating the role of glutamate receptors in neurological disorders
  • Exploring the mechanisms of synaptic transmission and plasticity

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