HOMER3 Human

Homer Homolog-3 Human Recombinant
Cat. No.
BT22417
Source
E.coli.
Synonyms
Homer homolog 3 (Drosophila)1 2 , HOMER-31 2 , homer protein homolog 32 , Homer neuronal immediate early gene 32, Homer-33, VESL32.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

HOMER3 Human Recombinant produced in E. coli is a single polypeptide chain containing 386 amino acids (1-361) and having a molecular mass of 42.5kDa.
HOMER3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
HOMER3, a member of the HOMER family of postsynaptic density scaffolding proteins, shares a conserved domain structure with its counterparts. This structure includes an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 (EVH1) domain, facilitating protein-protein interactions, along with two leucine zipper motifs and a C-terminal coiled-coil domain, both contributing to self-oligomerization.
Description
Recombinant human HOMER3, expressed in E. coli, is a single polypeptide chain encompassing 386 amino acids (residues 1-361) with a molecular weight of 42.5 kDa. The protein is appended with a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution, sterilized by filtration.
Formulation
The provided HOMER3 solution (1 mg/mL) is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM DTT, and 30% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the protein is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Homer homolog 3 (Drosophila)1 2 , HOMER-31 2 , homer protein homolog 32 , Homer neuronal immediate early gene 32, Homer-33, VESL32.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMMSTAR EQPIFSTRAH VFQIDPATKR NWIPAGKHAL TVSYFYDATR NVYRIISIGG AKAIINSTVT PNMTFTKTSQ KFGQWADSRA NTVYGLGFAS EQHLTQFAEK FQEVKEAARL AREKSQDGGE LTSPALGLAS HQVPPSPLVS ANGPGEEKLF RSQSADAPGP TERERLKKML SEGSVGEVQW EAEFFALQDS NNKLAGALRE ANAAAAQWRQ QLEAQRAEAE RLRQRVAELE AQAASEVTPT GEKEGLGQGQ SLEQLEALVQ TKDQEIQTLK SQTGGPREAL EAAEREETQQ KVQDLETRNA ELEHQLRAME RSLEEARAER ERARAEVGRA AQLLDVSLFE LSELREGLAR LAEAAP

Product Science Overview

Structure and Function

HOMER3 shares a similar domain structure with other members of the Homer family. It consists of:

  • An N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 (EVH1) domain, which mediates protein-protein interactions.
  • A carboxy-terminal coiled-coil domain and two leucine zipper motifs, which are involved in self-oligomerization .

These structural features enable HOMER3 to interact with various proteins and contribute to the formation and maintenance of synaptic structures. HOMER3 is particularly important in the postsynaptic density (PSD) of neurons, where it helps regulate the size and strength of synapses .

Role in Synaptic Plasticity

Synaptic plasticity is the ability of synapses to strengthen or weaken over time in response to changes in activity. This process is essential for learning, memory, and overall brain function. HOMER3, along with other Homer proteins, plays a key role in modulating synaptic plasticity by:

  • Regulating calcium signaling: HOMER3 interacts with metabotropic glutamate receptors (mGluRs) and inositol trisphosphate receptors (IP3Rs), which are involved in calcium signaling pathways .
  • Maintaining synaptic integrity: By interacting with various synaptic proteins, HOMER3 helps maintain the structural integrity of synapses and ensures proper synaptic function .
Expression and Localization

HOMER3 is expressed in various tissues, including the brain, heart, kidney, muscle, ovary, and testicles . In the brain, it is predominantly localized in the postsynaptic density of neurons, where it plays a critical role in synaptic function and plasticity .

Recombinant HOMER3

Recombinant HOMER3 is a form of the protein that is produced using recombinant DNA technology. This involves inserting the gene encoding HOMER3 into a host organism, such as E. coli, which then produces the protein. Recombinant HOMER3 is often used in research to study the protein’s structure, function, and interactions with other proteins .

The recombinant human HOMER3 protein typically includes a His-tag at the N-terminus to facilitate purification and detection. It is expressed in E. coli and purified using conventional chromatography techniques .

Applications in Research

Recombinant HOMER3 is used in various research applications, including:

  • Studying synaptic plasticity: Researchers use recombinant HOMER3 to investigate its role in synaptic plasticity and its interactions with other synaptic proteins.
  • Understanding neurological disorders: Dysregulation of Homer proteins, including HOMER3, has been implicated in various neurological disorders, such as autism, anxiety, and addiction . Studying recombinant HOMER3 can provide insights into the mechanisms underlying these conditions.
  • Drug development: By understanding the role of HOMER3 in synaptic function, researchers can develop targeted therapies for neurological disorders that involve dysregulation of synaptic plasticity .

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