HMGB1 Human, Sf9
Sf9 Insect Cells.
Description
HMG1 Human Recombinant fused to an 8 aa His-Tag at C-terminus produced in baculovirus insect cells is a single, glycosylated, polypeptide chain (amino acids 1-215) containing 223 amino acids and having a molecular mass of 25kDa. The HMGB1 is purified by proprietary chromatographic techniques.
Product Specs
Sf9 Insect Cells.
Product Science Overview
High-Mobility Group Box 1 (HMGB1) is a highly conserved, non-histone chromatin-binding protein found in the nucleus of eukaryotic cells. It plays a crucial role in the regulation of DNA-dependent processes such as transcription, replication, and repair. HMGB1 is also known for its role as a cytokine, influencing various cellular processes outside the nucleus.
HMGB1 consists of 215 amino acids and has a molecular mass of approximately 25 kDa . It contains two DNA-binding domains known as HMG-boxes and an acidic C-terminal tail. The protein can bind to DNA in a non-sequence-specific manner, facilitating the bending and looping of DNA, which is essential for the formation of nucleoprotein complexes .
Inside the cell, HMGB1 binds to DNA and is involved in transcriptional regulation. It acts as an architectural factor, stabilizing nucleoprotein complexes and facilitating the interaction of transcription factors with their target DNA sequences . Outside the cell, HMGB1 functions as a cytokine with activities resembling those of tumor necrosis factor (TNF). It can activate monocytes and neutrophils, playing a significant role in inflammation and immune responses .
Recombinant HMGB1 is produced using the baculovirus expression system in Sf9 insect cells. This method allows for the production of a glycosylated form of the protein, which closely resembles the native protein found in human cells . The recombinant HMGB1 is fused to an 8 amino acid His-Tag at the C-terminus, facilitating its purification through chromatographic techniques .
The recombinant HMGB1 (Human, Sf9) is available in various quantities and is shipped with ice packs to maintain its stability. It is formulated as a sterile, filtered, colorless solution containing 20mM Tris-HCl (pH 8), 1mM EDTA, 0.5mM DTT, and 10% glycerol . The protein is stable at 4°C for short-term storage and at -20°C for long-term storage, with the addition of a carrier protein to prevent multiple freeze-thaw cycles .
HMGB1 has a wide range of applications in research and clinical settings. It is used to study chromatin dynamics, transcriptional regulation, and DNA repair mechanisms. Additionally, HMGB1 is involved in various pathological conditions, including cancer, sepsis, and autoimmune diseases . Its role as a cytokine makes it a target for therapeutic interventions aimed at modulating immune responses and inflammation.
