HMGB1 Antibody

High-Mobility Group Box 1, Mouse Anti Human
Cat. No.
BT20125
Source
Synonyms
HMG1, HMG3, SBP-1, Amphoterin, HMGB1, High-Mobility Group Box 1.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin, belongs to the high mobility group box family of non-histone chromosomal proteins. The human HMGB1 protein is a 30 kDa, 215 amino acid (aa) single chain polypeptide composed of three domains: two positively charged DNA-binding domains at the N-terminal (HMG boxes A and B) consisting of 70 aa each, and a negatively charged C-terminal region of 30 aa containing only Asp and Glu residues. The protein contains a nuclear localization signal (NLS) within residues 27-43 and 178-184. Posttranslational modifications, such as acetylation of up to 17 lysine residues, have been observed. HMGB1 is abundantly expressed in virtually all cell types. Initially identified as a nuclear protein capable of inducing DNA bending, HMGB1 contributes to nucleosome stability and regulates gene expression through interactions with DNA-binding proteins.
Formulation
Supplied as a 1mg/ml solution in PBS, pH 7.4, with 0.1% Sodium Azide.
Storage Procedures
Store at 4°C for periods up to 1 month. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
The HMGB1 antibody has been validated for ELISA, Western blot, and immunohistochemistry, demonstrating its specificity and reactivity. However, optimization of the reagent concentration is recommended for individual experimental conditions. The recommended dilution range for Western blot analysis is 1:500 to 1:2,000, with a starting dilution of 1:1,000. For immunohistochemistry, a dilution range of 1:100 to 1:300 is suggested, with an initial dilution of 1:200.
Synonyms
HMG1, HMG3, SBP-1, Amphoterin, HMGB1, High-Mobility Group Box 1.
Purification Method
HMGB1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PJ2E1AT.
Immunogen
Anti-human HMGB1 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HMGB1 amino acids 1-215 purified from High Five (Trichoplusia ni) insect cells.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Introduction

High-Mobility Group Box 1 (HMGB1) is a non-histone chromatin-associated protein that plays a crucial role in various cellular processes and disease mechanisms. It is a highly conserved protein found in the nucleus of eukaryotic cells and is involved in DNA binding, transcriptional regulation, and chromatin remodeling .

Structure and Function

HMGB1 consists of 215 amino acids and has a molecular weight of approximately 30 kDa. It is composed of two DNA-binding domains (HMG boxes A and B) and an acidic C-terminal tail. The protein can bind to DNA and bend it, facilitating the assembly of nucleoprotein complexes .

Intracellularly, HMGB1 is involved in:

  • Transcriptional regulation: It acts as a co-factor for various transcription factors.
  • DNA repair: It participates in the recognition and repair of damaged DNA.
  • Chromatin remodeling: It helps in the structural organization of chromatin.

Extracellularly, HMGB1 can be released into the extracellular space during cell stress, injury, or necrosis. In this context, it acts as a damage-associated molecular pattern (DAMP) molecule, triggering inflammatory responses by binding to pattern recognition receptors (PRRs) such as TLR4 and RAGE .

Role in Disease

HMGB1 is implicated in various diseases, including:

  • Cancer: It promotes tumor growth, metastasis, and resistance to chemotherapy.
  • Inflammatory diseases: It is involved in the pathogenesis of conditions like rheumatoid arthritis and sepsis.
  • Neurological disorders: It contributes to the inflammatory response in diseases such as stroke and spinal cord injury .
Mouse Anti-Human HMGB1 Antibody

Mouse anti-human HMGB1 antibodies are monoclonal antibodies developed to specifically target and neutralize human HMGB1. These antibodies are used in research and therapeutic applications to study and modulate the role of HMGB1 in various diseases .

Applications
  • Research: These antibodies are used to investigate the biological functions of HMGB1 and its role in disease mechanisms.
  • Therapeutics: Anti-HMGB1 antibodies have shown potential in treating conditions like spinal cord injury, where they help reduce inflammation and promote functional recovery .

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THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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