HIV-1 p24 Paired
Greater than 95%.
Description
HIV-1 p24 Paired antibodies coating and conjugating are used for lateral flow immunoassay and having a Mw of 24kDa.
Please note that when ordering for example: 100µg antibody we ship 50µg from each of the antibodies (100µg in total).
Product Specs
The human immunodeficiency virus (HIV) belongs to the Retroviridae family and Lentivirus genus, known for causing long-duration illnesses with extended incubation periods. HIV is a retrovirus that primarily targets vital immune system cells, including CD4+ T cells, macrophages, and dendritic cells. The virus compromises the immune system, making individuals susceptible to opportunistic infections. HIV transmission occurs through bodily fluids, leading to the infection of target cells. The virus inserts its RNA genome into the host cell's DNA, establishing either a latent or active infection. Active infection results in viral replication and the release of new virus particles. Notably, the p24 protein, detectable in blood shortly after infection, serves as an early indicator of HIV infection.
These HIV-1 p24 Paired antibodies are designed for lateral flow immunoassays and have a molecular weight of 24kDa. One antibody is intended for coating, while the other is for conjugation purposes. When ordering, for instance, 100µg of antibody, you will receive two vials: one containing 50µg of the coating antibody and the other containing 50µg of the conjugating antibody, totaling 100µg.
Exceeds 95% purity.
The HIV-1 p24 coating antibody is prepared in phosphate-buffered saline (PBS) at a pH of 7.4. Similarly, the HIV-1 p24 conjugating antibody is also provided in PBS at a pH of 7.4.
While the HIV-1 p24 antibody remains stable at 4°C for a week, it is recommended to store it at temperatures below -18°C. For long-term storage, adding a carrier protein like HSA or BSA (0.1%) is advisable. It is essential to avoid repeated freeze-thaw cycles to maintain antibody stability.
These antibodies are specifically suitable for use in lateral flow immunoassays.
Purified by protein A affinity column.
Product Science Overview
The Human Immunodeficiency Virus type 1 (HIV-1) is a retrovirus responsible for the acquired immunodeficiency syndrome (AIDS). One of the key components of HIV-1 is the p24 protein, also known as the capsid protein. This protein plays a crucial role in the virus’s structure and lifecycle. The detection of p24 is essential for diagnosing and monitoring HIV-1 infections. Mouse anti-HIV-1 p24 paired antibodies are widely used in various immunoassays to detect the presence of the p24 protein.
The p24 protein is a part of the Gag polyprotein, which is cleaved by the viral protease to form the mature capsid protein. The p24 protein is approximately 24 kDa in size and forms the conical core of the virus. It is highly conserved among different HIV-1 strains, making it an ideal target for diagnostic assays. The presence of p24 in the blood can be detected earlier than antibodies against HIV-1, making it a valuable marker for early diagnosis.
Mouse anti-HIV-1 p24 antibodies are monoclonal antibodies produced by immunizing mice with the p24 protein or its fragments. These antibodies are highly specific to the p24 protein and are used in various immunoassays, including enzyme-linked immunosorbent assay (ELISA), Western blot, immunohistochemistry (IHC), and immunofluorescence (IF). The paired antibodies typically consist of a capture antibody and a detection antibody, which work together to detect the p24 protein with high sensitivity and specificity.
- ELISA: In ELISA, the capture antibody is coated onto a microplate, and the sample is added. If p24 is present, it binds to the capture antibody. The detection antibody, conjugated to an enzyme such as horseradish peroxidase (HRP), is then added. The enzyme catalyzes a colorimetric reaction, allowing for the quantification of p24.
- Western Blot: In Western blot, proteins from a sample are separated by gel electrophoresis and transferred to a membrane. The membrane is then probed with the mouse anti-HIV-1 p24 antibodies. The detection antibody, conjugated to an enzyme or a fluorescent dye, allows for the visualization of the p24 protein.
- Immunohistochemistry (IHC): In IHC, tissue sections are stained with the mouse anti-HIV-1 p24 antibodies to detect the presence of p24 in tissue samples. This technique is useful for studying the distribution and localization of the virus in infected tissues.
- Immunofluorescence (IF): In IF, cells or tissue sections are stained with the mouse anti-HIV-1 p24 antibodies conjugated to a fluorescent dye. This allows for the visualization of the p24 protein under a fluorescence microscope.
- High Specificity: Mouse anti-HIV-1 p24 antibodies are highly specific to the p24 protein, reducing the likelihood of cross-reactivity with other proteins.
- Early Detection: The p24 protein can be detected earlier than antibodies against HIV-1, making these antibodies valuable for early diagnosis.
- Versatility: These antibodies can be used in various immunoassays, providing flexibility in experimental design.
