HIV1 p24 Antibody, C-80
Sterile Filtered White lyophilized (freeze-dried) powder.
Description
Product Specs
The antibody appears as a sterile filtered white powder in a lyophilized (freeze-dried) state.
The antibody has been lyophilized using a phosphate-buffered saline (PBS) solution.
The antibody is shipped in its lyophilized state at ambient temperature.
The lyophilized antibody should be stored long-term at 4°C in a dry environment. Upon reconstitution, any unused antibody should be aliquoted and stored at -20°C for future use. This storage method helps to maintain the antibody's stability and activity.
To reconstitute the lyophilized antibody, add 1ml of 1x PBS to the vial. Gently mix the solution, ensuring the PBS washes down any antibody adhered to the vial's sides. Allow 30-60 seconds for complete reconstitution before use.
Protein A / S-Sepharose.
Mouse Monoclonal antibodies against HIV-1 full length recombinant p24. Captures the p24 antigen. May be paired with mAbs catalog numbers ant-747.
80
r.HIV-1p24 Core.
mouse IgG1.
Product Science Overview
HIV-1 p24 is a capsid protein that forms the core of the Human Immunodeficiency Virus type 1 (HIV-1). It is produced by the cleavage of the Gag polyprotein precursor (p55) by the HIV-1 protease. The p24 protein is crucial for the assembly and maturation of the virus, making it an essential target for diagnostic and research purposes.
The HIV-1 p24 protein is a part of the Gag polyprotein, which also includes matrix (p17), nucleocapsid (p7), and other smaller proteins. The p24 protein is approximately 24 kDa in size and is highly conserved among different HIV-1 strains. It plays a vital role in the viral life cycle, including the formation of the viral core, encapsulation of the viral RNA, and interaction with host cell factors.
The p24 protein is detectable from the early stages of HIV infection and is used as a marker for monitoring disease progression and treatment efficacy. It is also employed in various research applications to study the biology of HIV-1 and to develop new therapeutic strategies.
The Mouse Antibody, Clone-80, is a monoclonal antibody specifically developed to target the HIV-1 p24 protein. Monoclonal antibodies are produced by identical immune cells that are clones of a unique parent cell, ensuring specificity and uniformity in their action.
Characteristics
- Host Species: Mouse
- Isotype: IgG1
- Clone: 80
- Applications: The Clone-80 antibody can be used in several scientific applications, including Western Blot (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Immunocytochemistry (ICC), and Immunohistochemistry (IHC).
Production and Purification
The Clone-80 antibody is produced by immunizing mice with a recombinant HIV-1 p24 protein. The antibody-producing cells are then fused with myeloma cells to create hybridomas, which are screened for the production of the desired antibody. The selected hybridomas are cultured, and the antibodies are purified from the culture supernatant using Protein G affinity chromatography.
The HIV-1 p24 (Mouse Antibody, Clone-80) is widely used in research to:
- Detect HIV-1 p24: In various assays to monitor viral load and study the virus’s replication cycle.
- Study Viral Assembly: To understand the role of p24 in the assembly and maturation of HIV-1 particles.
- Develop Therapeutics: To screen for potential inhibitors that target the p24 protein and disrupt the viral life cycle.
