HAV VP4-VP2

Hepatitis A Virus VP4-VP2 Recombinant
Cat. No.
BT8933
Source
Escherichia Coli.
Synonyms
Appearance
Purity
HAV VP4-VP2 protein is >90% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

The E.Coli derived 44 kDa recombinant protein fused to a GST tag contains the VP4-VP2 immunodominant regions, amino acids 55-164.

Product Specs

Introduction
Hepatitis A virus (HAV) possesses forty-two antigenic domains across its polyprotein, as revealed by analysis using 237 overlapping synthetic peptides (20-mers) covering the entire HAV polyprotein. Nineteen of these domains reside within structural proteins, while 22 are located within nonstructural proteins, with one domain spanning the junction of the VP1 and P2A proteins. Five domains were identified as immunodominant based on their breadth and strength of immunoreactivity. The first immunodominant domain is situated within the VP2 protein at amino acid positions 57-90. The second domain, spanning amino acid positions 767-842, encompasses the C-terminal portion of the VP1 protein and the entire P2A protein. The third domain, located at amino acid positions 1403-1456, includes the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain, situated at amino acid positions 1500-1519, spans nearly the entire P3B protein. The fifth and final immunodominant domain, located at amino acid positions 1719-1764, comprises the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. Notably, four of the five most immunoreactive domains originate from small HAV proteins and/or encompass protein cleavage sites that separate distinct HAV proteins.
Description
This recombinant protein, expressed in E. coli, is a 44 kDa protein consisting of the HAV VP4-VP2 immunodominant regions (amino acids 55-164) fused to a GST tag.
Purity
The purity of the HAV VP4-VP2 protein is greater than 90% as determined by 10% SDS-PAGE and Coomassie blue staining.
Formulation
The protein is supplied in a buffer containing 10mM CBB (Coomassie Brilliant Blue), pH 9.6, 0.1% SDS (Sodium Dodecyl Sulfate), and 50% glycerol.
Stability
For optimal stability, HAV VP4-VP2 protein should be stored at -18°C. While the protein remains stable at 4°C for up to 1 week, it is recommended to store it at -18°C for long-term storage. Avoid repeated freeze-thaw cycles.
Applications
HAV VP4-VP2 antigen is suitable for use in ELISA and Western blotting applications. It is an excellent antigen for the detection of HAV with minimal specificity issues.
Source
Escherichia Coli.
Purification Method

HAV VP4-VP2 Protein was purified by proprietary chromatographic technique.

Specificity
Immunoreactive with sera HAV-infected individuals.

Product Science Overview

Introduction

Hepatitis A Virus (HAV) is a member of the Picornaviridae family, known for causing acute hepatitis in humans. The virus has a positive-sense RNA genome encapsidated in a shell formed by 60 copies of at least three viral proteins: VP1, VP2, and VP3 . A small structural protein, VP4, plays a crucial role in the assembly and stability of the viral capsid .

Structural Proteins of HAV

The HAV capsid is composed of four structural proteins: VP1, VP2, VP3, and VP4 . These proteins are essential for the formation and stability of the viral particle. The mature icosahedral capsid is composed of 12 pentamers, each consisting of five copies of these four proteins . The smallest of these proteins, VP4, is located on the inside of the capsid shell, facing the viral RNA genome .

Role of VP4 and VP2 in Capsid Assembly

The assembly of the HAV capsid is a complex process that involves the proteolytic processing of a precursor polyprotein. VP4 and VP2 play distinct roles in this process . VP4 is liberated during the final step of capsid maturation and is found on the inside of the capsid shell . The myristoylated N-terminus of VP4 is located below the 5-fold vertices, while its C-terminus is found at the 3-fold axis . VP2, on the other hand, interacts with VP3 and neighboring protomers at the 2-fold axis .

Recombinant VP4-VP2

Recombinant VP4-VP2 refers to the use of recombinant DNA technology to produce these proteins in a laboratory setting. This approach allows researchers to study the individual roles of these proteins in capsid assembly and stability. By expressing VP4 and VP2 recombinantly, scientists can investigate their interactions and the signals required for the assembly of viral particles .

Applications of Recombinant VP4-VP2

Understanding the roles of VP4 and VP2 in HAV capsid assembly has several important applications:

  1. Vaccine Development: Insights into the assembly process can aid in the design of empty capsid-based recombinant vaccines .
  2. Drug Delivery Systems: Knowledge of capsid assembly can be leveraged to develop drug delivery systems that mimic viral particles .
  3. Pathogenesis Studies: Studying the interactions of recombinant VP4 and VP2 can provide valuable information about the pathogenesis of HAV and potential therapeutic targets .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

HAV P2C
Hepatitis A Virus P2C Recombinant
Cat. No.
BT8287
Source
HAV P2C-P3A
Hepatitis A Virus P2C-P3A Recombinant
Cat. No.
BT8374
Source
Escherichia Coli.
HAV VP1-P2A (722-830 a.a.)
Hepatitis A Virus VP1-P2A (722-830 a.a.) Recombinant
Cat. No.
BT8701
Source
HAV VP3
Hepatitis A Virus VP3 Recombinant
Cat. No.
BT8849
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.