HAV P2C-P3A

Hepatitis A Virus P2C-P3A Recombinant
Cat. No.
BT8374
Source
Escherichia Coli.
Synonyms
Appearance
Purity
HAV P2C-P3A protein is >90% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.Coli derived recombinant protein contains the P2C-P3A immunodominant regions, amino acids 1392-1521.

Product Specs

Introduction
Hepatitis A virus (HAV) possesses numerous antigenic domains within its polyprotein structure. Research utilizing 237 overlapping synthetic peptides has revealed 42 such domains, with 19 located in structural proteins and 22 in nonstructural proteins. Notably, one domain spans the VP1 and P2A protein junction. Five of these domains exhibit immunodominance due to their broad and strong immunoreactivity. These key domains include one within VP2 protein (57-90 aa), another encompassing the C-terminal VP1 and the entire P2A protein (767-842 aa), a third covering the C-terminal P2C and N-terminal half of P3A protein (1403-1456 aa), a fourth spanning almost all of P3B (1500-1519 aa), and a final domain containing the C-terminal P3C and N-terminal P3D protein regions (1719-1764 aa). Interestingly, four of these highly immunoreactive domains originate from smaller HAV proteins or encompass protein cleavage sites.
Description
This recombinant protein, derived from E. coli, encompasses the immunodominant regions of HAV P2C-P3A, specifically amino acids 1392-1521.
Purity
The purity of HAV P2C-P3A protein exceeds 90%, as determined by 10% PAGE (coomassie staining).
Formulation
The protein is formulated in a solution of 10mM CBB, pH 9.6, containing 0.1% SDS and 50% glycerol.
Stability
For optimal stability, HAV P2C-P3A protein should be stored below -18°C. While it can remain stable at 4°C for up to one week, it's important to avoid repeated freeze-thaw cycles.
Applications
HAV P2C-P3A antigen serves as a valuable tool in various research applications, including ELISA and Western blots. Its ability to effectively detect HAV with minimal specificity issues makes it an excellent antigen choice.
Source
Escherichia Coli.
Purification Method
HAV P2C-P3A protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera HAV-infected individuals.

Product Science Overview

Introduction

Hepatitis A virus (HAV) is a non-enveloped, positive-stranded RNA virus belonging to the genus Hepatovirus in the family Picornaviridae . It poses a significant public health threat, causing acute liver disease known as hepatitis A. The HAV genome consists of a large open reading frame, which includes the P1, P2, and P3 regions . The P2 and P3 regions encode non-structural proteins essential for viral replication and assembly.

P2C and P3A Proteins

The P2C protein is a putative NTPase, which plays a pivotal role in viral replication . It is involved in membrane association and RNA binding, crucial for the replication process. The P3A protein, on the other hand, is involved in the assembly and release of the virus. Together, these proteins are essential for the life cycle of HAV.

Recombinant Hepatitis A Virus P2C-P3A

Recombinant HAV P2C-P3A is a synthetic construct that combines the P2C and P3A proteins. This recombinant protein is used in various research and diagnostic applications. It is manufactured with high sensitivity, specificity, and reproducibility, making it ideal for use in immunization protocols and generating antibodies that specifically target the protein of interest .

Applications

The recombinant HAV P2C-P3A protein is widely used in research to study the mechanisms of HAV replication and assembly. It is also used in the development of diagnostic assays and vaccines. The high specificity and sensitivity of this recombinant protein make it a valuable tool in the field of virology and immunology.

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