HAV VP3

Hepatitis A virus (HAV) is a significant cause of infectious hepatitis worldwide, primarily transmitted through the fecal-oral route. The virus is a member of the Picornaviridae family and has a positive-sense single-stranded RNA genome. The viral capsid is composed of three main proteins: VP1, VP2, and VP3. The VP3 protein plays a crucial role in the virus’s structure and function, making it a target for recombinant protein production and research.

The preparation of recombinant VP3 protein involves several steps, starting with the isolation of the VP3 gene from the HAV genome. This gene is then cloned into an appropriate expression vector, such as the pTOP Blunt V2 vector. The recombinant plasmid DNA is isolated and evaluated for concentration using UV absorbance measurements . The expression of the VP3 protein is typically carried out in a bacterial system, such as Escherichia coli, where the protein is induced and purified using affinity chromatography techniques .

The biochemical characterization of recombinant VP3 protein involves various assays to determine its activity and interactions. For instance, the interaction of HAV with its cellular receptor, HAVCR1, has been studied to understand the infectivity and entry process of the virus . Additionally, the VP3 protein’s role in mediating host cell interactions and its involvement in viral replication and assembly are critical areas of research . The analysis of these interactions provides insights into the virus’s life cycle and potential targets for antiviral therapies.