HAV P2C
Description
Product Specs
For optimal stability, HAV P2C protein should be stored at -18°C. While it can remain stable at 4°C for up to one week, long-term storage at this temperature is not recommended.
Repeated freezing and thawing of the protein should be avoided.
Product Science Overview
Hepatitis A virus (HAV) is a significant pathogen causing communicable liver disease worldwide. It belongs to the family Picornaviridae and is the sole member of the Hepatovirus genus . HAV infection is generally mild, but in some cases, particularly in older adults, it can lead to severe liver disease, including fulminant hepatitis .
HAV is a non-enveloped virus with an icosahedral capsid approximately 27 nm in diameter . The virus has a single-stranded RNA genome of about 7.5 kb, which encodes a single polyprotein that is processed into structural and nonstructural proteins . The nonstructural proteins are essential for viral replication and include the 2C protein, which is the focus of this article.
The 2C protein of HAV is a nonstructural protein that plays a crucial role in viral replication . It shares 24-27% sequence identity with 2C proteins of other picornaviruses, and key motifs are conserved . The 2C protein has been shown to possess ATPase activity but lacks helicase activity . Additionally, it exhibits an ATPase-independent ribonuclease activity with a preference for polyuridylic single-stranded RNAs .
Recombinant HAV 2C protein has been studied to understand its biochemical and structural properties. The crystal structure of an HAV 2C fragment has been determined to a resolution of 2.2 Å, revealing an ATPase domain, a region equivalent to the enterovirus 2C zinc-finger (ZFER), and a C-terminal amphipathic helix (PBD) . The PBD of HAV 2C occupies a hydrophobic pocket in the adjacent 2C, and this interaction is vital for 2C functions .
Recombinant HAV genomes containing foreign protein-coding sequences inserted at the 2A/2B junction are novel and potentially useful protein expression vectors . These recombinant genomes can tolerate insertions and have been used to study the functions of various viral proteins . Understanding the biochemical and structural properties of HAV 2C can aid in the development of effective anti-HAV drugs and improve disease control measures .
