HAV VP1-P2A (722-830 a.a.)

Hepatitis A Virus VP1-P2A (722-830 a.a.) Recombinant
Cat. No.
BT8701
Source
Synonyms
Appearance
Purity
HAV VP1-P2A protein is >90% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.Coli derived 51.2 kDa recombinant protein fused to a GST tag contains the VP1-P2A immunodominant regions, amino acids 722-830.

Product Specs

Introduction
Hepatitis A virus (HAV) possesses forty-two antigenic domains across its polyprotein. These were identified using 237 overlapping synthetic peptides (20-mers) covering the entire HAV polyprotein. Nineteen antigenic domains are located within the structural proteins, while 22 are within the nonstructural proteins, with one domain spanning the VP1 and P2A protein junction. Five domains are considered immunodominant due to their broad and strong immunoreactivity. One domain is located in the VP2 protein (amino acids 57-90). A second domain (amino acids 767-842) includes the VP1 protein's C-terminus and the complete P2A protein. A third domain (amino acids 1403-1456) encompasses the P2C protein's C-terminus and the P3A protein's N-terminal half. The fourth domain (amino acids 1500-1519) covers almost the entire P3B protein, and the fifth domain (amino acids 1719-1764) includes the P3C protein's C-terminal region and the P3D protein's N-terminal region. Notably, four of the five most immunoreactive domains originate from small HAV proteins or span protein cleavage sites between different HAV proteins.
Description
This recombinant protein, expressed in E. coli, is 51.2 kDa in size and consists of the HAV VP1-P2A immunodominant regions (amino acids 722-830) fused to a GST tag.
Purity
The purity of the HAV VP1-P2A protein exceeds 90%, as determined by 10% SDS-PAGE and Coomassie blue staining.
Formulation
The protein is supplied in a buffer containing 10mM CBB (Coomassie Brilliant Blue), pH 9.6, 0.1% SDS (Sodium Dodecyl Sulfate), and 50% glycerol.
Stability
For optimal stability, store the HAV VP1-P2A protein at -18°C or below. While it remains stable at 4°C for up to one week, repeated freeze-thaw cycles should be avoided.
Applications
The HAV VP1-P2A antigen is ideal for use in ELISA and Western blots. It serves as an excellent antigen for the detection of HAV with minimal cross-reactivity.
Purification Method
Inclusion bodies.
Specificity
Immunoreactive with sera HAV-infected individuals.

Product Science Overview

Introduction

Hepatitis A Virus (HAV) is a significant cause of infectious hepatitis worldwide, primarily transmitted through the fecal-oral route. The virus’s genome consists of a 7,500-nucleotide linear, positive-stranded RNA . The VP1-P2A region of the HAV polyprotein is particularly noteworthy due to its immunogenic properties and its role in the virus’s structural integrity.

Structure and Function

The VP1-P2A region spans amino acids 722 to 830 of the HAV polyprotein. This region includes the C-terminal part of the VP1 protein and the entire P2A protein . The VP1 protein is a coat protein, while P2A is a core protein. Together, they form a crucial part of the virus’s structure and are involved in its ability to infect host cells.

Immunogenic Properties

The VP1-P2A region is highly immunogenic, meaning it can elicit a strong immune response. This property makes it an excellent target for vaccine development and diagnostic assays. Studies have identified several antigenic domains within the HAV polyprotein, with the VP1-P2A region being one of the most immunoreactive . This region is particularly useful in ELISA and Western blot assays for detecting HAV infections with minimal specificity problems .

Recombinant Expression

Recombinant HAV VP1-P2A protein is typically expressed in Escherichia coli (E. coli) or Lactococcus lactis . The recombinant protein is purified using chromatographic techniques to achieve a purity of over 90% . The protein is often fused to a GST tag to facilitate purification and detection. The recombinant protein is stable at -20°C and should be stored to avoid freeze-thaw cycles .

Applications

The recombinant HAV VP1-P2A protein has several applications in research and diagnostics:

  • Vaccine Development: Due to its strong immunogenic properties, the VP1-P2A region is a promising candidate for vaccine development .
  • Diagnostic Assays: The protein is used in ELISA and Western blot assays to detect HAV infections .
  • Research: The recombinant protein is used in various research studies to understand the virus’s structure, function, and immune response mechanisms .

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