HAV VP1-P2A (669-782 a.a)

Hepatitis A Virus VP1-P2A (669-782 a.a.) Recombinant
Cat. No.
BT8610
Source
Escherichia Coli.
Synonyms
Appearance
Purity
HAV VP1-P2A protein is >90% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.Coli derived recombinant protein contains the VP1-P2A immunodominant regions, amino acids 669-782.

Product Specs

Introduction
Forty-two antigenic domains of the hepatitis A virus (HAV) polyprotein were identified using 237 overlapping synthetic peptides, each 20 amino acids long, covering the entire HAV polyprotein. Nineteen antigenic domains were within the structural proteins, and 22 were within the nonstructural proteins, with one domain spanning the VP1 and P2A protein junction. Five domains were classified as immunodominant based on their strong immunoreactivity and recognition by a wide range of antibodies. One domain is located in the VP2 protein between amino acids 57 and 90. A second domain, spanning amino acids 767 to 842, includes the C-terminal part of the VP1 protein and the entire P2A protein. A third domain, located between amino acids 1403 and 1456, comprises the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain, located between amino acids 1500 and 1519, includes almost all of P3B. The final domain, located between amino acids 1719 and 1764, contains the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. Notably, four of the five most immunoreactive domains come from small HAV proteins and/or include protein cleavage sites that separate different HAV proteins.
Description
This recombinant protein is derived from E. coli and contains the immunodominant regions of the HAV VP1-P2A protein, specifically amino acids 669 to 782.
Purity
The purity of the HAV VP1-P2A protein exceeds 90%, as determined by 10% SDS-PAGE and Coomassie blue staining.
Formulation
The HAV VP1-P2A protein is supplied in a buffer consisting of 10 mM Tris-HCl (pH 9.6), 1.5 M urea, and 50% glycerol.
Stability
For optimal stability, store the HAV VP1-P2A protein below -18°C. While it remains stable at 4°C for up to one week, it is recommended to avoid repeated freeze-thaw cycles.
Applications
The HAV VP1-P2A antigen is suitable for use in ELISA and Western blot assays. Its high specificity for HAV detection minimizes cross-reactivity with other antigens, making it an excellent choice for HAV detection.
Source
Escherichia Coli.
Purification Method
HAV VP1-P2A protein was purified by proprietary chromatographic technique.
Specificity
Immunoreactive with sera HAV-infected individuals.

Product Science Overview

Introduction

Hepatitis A Virus (HAV) is a positive-stranded RNA virus belonging to the genus Hepatovirus in the family Picornaviridae . It is a significant cause of infectious hepatitis worldwide, primarily transmitted through the fecal-oral route . The virus has a single-stranded RNA genome that encodes a polyprotein, which is subsequently cleaved into structural and non-structural proteins.

Structural Proteins and Antigenic Domains

The HAV polyprotein contains several antigenic domains, which are regions recognized by the immune system . Among these, the VP1-P2A region is particularly notable. The VP1 protein is a major capsid protein that plays a crucial role in the assembly and stability of the viral particle . The P2A protein is a non-structural protein involved in the viral replication process .

Recombinant VP1-P2A Protein

The recombinant VP1-P2A protein, specifically the region spanning amino acids 669-782, is produced using heterologous expression systems such as Escherichia coli . This recombinant protein includes immunodominant regions that are highly reactive with sera from HAV-infected individuals . The production of recombinant proteins is essential for developing subunit vaccines and diagnostic tools, as it allows for the generation of viral proteins without the need for live virus cultures .

Applications and Importance

The recombinant VP1-P2A protein has several applications in biomedical research and public health. It is used in enzyme-linked immunosorbent assays (ELISA) and Western blotting to detect HAV infections . Additionally, the recombinant protein can be utilized to study the molecular mechanisms of HAV capsid assembly and to develop new vaccines and therapeutic strategies .

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