GRO b Rat
Description
The GRO-b is purified by proprietary chromatographic techniques.
Product Specs
VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN.
Product Science Overview
GRO-beta/MIP-2 was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation . The protein and DNA sequences of MIP-2 revealed that it is a member of the alpha (C-X-C) subfamily of chemokines . The nomenclature “GRO-beta” refers to its growth-regulated oncogene properties, while “MIP-2” stands for macrophage inflammatory protein-2.
The recombinant rat CXCL2/GRO-beta/MIP-2 protein is a single, non-glycosylated polypeptide chain containing 73 amino acids . It is produced in Escherichia coli and purified to a high degree of purity, typically over 98% . The protein has a molecular weight of approximately 7.9 kDa .
CXCL2 functions primarily as a chemoattractant for neutrophils, guiding them to sites of infection or inflammation. It binds to the CXCR2 receptor on the surface of neutrophils, triggering a signaling cascade that results in the directed migration of these immune cells . This chemotactic activity is crucial for the rapid response of the immune system to bacterial infections and tissue damage.
The biological activity of recombinant rat CXCL2/GRO-beta/MIP-2 has been extensively studied. It is fully biologically active when compared to standard preparations, with its activity determined by a chemotaxis bioassay using rat neutrophils . The effective concentration range for this activity is typically between 10-100 ng/mL .
For optimal stability, the lyophilized recombinant protein should be stored at -20 to -70°C. Upon reconstitution, it is recommended to store the protein at 2-8°C for short-term use (up to one month) and at -20 to -70°C for long-term storage (up to several months). Repeated freeze-thaw cycles should be avoided to maintain the protein’s integrity and activity .
