GRO g Human, His
GRO-Gamma Human Recombinant (CXCL3), His Tag
Cat. No.
BT13958
Source
Escherichia Coli.
Synonyms
Macrophage inflammatory protein 2-beta, MIP2-beta, CXCL3, Growth-regulated protein gamma, GRO-gamma, GRO-gamma(1-73), GRO3, GROg, MIP2B, SCYB3, MIP-2b, CINC-2b, MGSA gamma.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
GRO-Gamma Human Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 94 amino acids (35-107) and having a molecular mass of 7902 Dalton. The GRO-g is fused to 20 amino acid His-Tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine known as GRO3 oncogene (GRO3), GRO protein gamma (GROg), and macrophage inflammatory protein-2-beta (MIP2b). It belongs to the CXC chemokine family. CXCL3 regulates monocyte migration and adhesion and exerts its effects by interacting with the CXCR2 chemokine receptor on target cells. The gene encoding CXCL3 is found on chromosome 4, clustered with other CXC chemokine genes.
Description
Recombinant human GRO-Gamma, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 94 amino acids (35-107). It has a molecular weight of 7902 Daltons. A 20 amino acid His-Tag is fused to the N-terminus of GRO-g, which is purified using proprietary chromatographic methods.
Physical Appearance
A sterile, colorless, and clear solution.
Formulation
The CXCL3 protein is supplied in a buffer consisting of 20mM Tris-HCl (pH 8), 1mM DTT, and 20% glycerol.
Stability
While liquid CXCL3 remains stable at 10°C for one week, it is recommended to store it below -18°C. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is advised. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95.0% using SDS-PAGE analysis.
Synonyms
Macrophage inflammatory protein 2-beta, MIP2-beta, CXCL3, Growth-regulated protein gamma, GRO-gamma, GRO-gamma(1-73), GRO3, GROg, MIP2B, SCYB3, MIP-2b, CINC-2b, MGSA gamma.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASVVTELRC QCLQTLQGIH LKNIQSVNVR SPGPHCAQTE VIATLKNGKK ACLNPASPMV QKIIEKILNKGSTN.
Product Science Overview
Introduction
GRO-Gamma, also known as Chemokine (C-X-C motif) ligand 3 (CXCL3), is a member of the CXC chemokine family. This family of proteins is known for its role in chemotaxis, where they guide the migration of immune cells to sites of inflammation or injury. The recombinant form of GRO-Gamma, tagged with a polyhistidine (His) tag, is produced for research and therapeutic purposes.
Structure and Expression
The recombinant human GRO-Gamma (CXCL3) protein is typically expressed in Escherichia coli (E. coli) systems. The protein consists of 91 amino acids and has a predicted molecular mass of approximately 10.1 kDa . The His tag, usually located at the N-terminus, facilitates purification and detection of the protein.
Biological Function
CXCL3 plays a crucial role in various physiological and pathological processes:
- Chemotaxis: It attracts neutrophils and basophils to sites of infection or inflammation .
- Inflammation: CXCL3 is involved in the inflammatory response and can exert its effects on endothelial cells in an autocrine manner .
- Cancer: It is implicated in the progression and metastasis of certain cancers, including prostate cancer .
- Adipogenesis: CXCL3 acts as an adipokine, promoting adipogenesis through autocrine and paracrine pathways .
Industrial Production
The production of recombinant GRO-Gamma involves several key steps:
- Gene Cloning: The gene encoding CXCL3 is cloned into an expression vector that includes a sequence for the His tag.
- Transformation: The vector is introduced into E. coli cells, which are then cultured under conditions that promote protein expression.
- Purification: The His-tagged protein is purified using affinity chromatography, which exploits the affinity between the His tag and nickel ions.
- Validation: The purity and activity of the recombinant protein are validated using techniques such as SDS-PAGE and functional assays .
Applications
Recombinant GRO-Gamma is used in various research and clinical applications:
- Cancer Research: Studying the role of CXCL3 in cancer progression and metastasis.
- Inflammation Studies: Investigating the mechanisms of inflammation and the role of chemokines.
- Drug Development: Screening potential therapeutic agents that target CXCL3 or its receptor, CXCR2.
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