GRO b Human, His
Greater than 90.0% as determined by SDS-PAGE.
Description
GRO-Beta Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 94 amino acids (35-107 a.a) and having a molecular mass of 10.1 kDa. The GRO-b is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
GRO-Beta, also known as CXCL2, is a chemokine belonging to the CXC chemokine family. Chemokines are small cytokines or signaling proteins secreted by cells that have the ability to induce directed chemotaxis in nearby responsive cells. CXCL2 is particularly known for its role in the immune system, where it functions as a chemoattractant for neutrophils.
The human recombinant GRO-Beta (CXCL2) is typically produced in E. coli and is often tagged with a His-tag to facilitate purification. The His-tag is a sequence of histidine residues that binds to nickel ions, allowing for easy separation of the protein from other cellular components using affinity chromatography.
The recombinant form of CXCL2 is a non-glycosylated polypeptide chain containing 73 amino acids . It has a molecular weight of approximately 7.9 kDa . The protein is lyophilized and can be reconstituted in sterile distilled water or an aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL .
CXCL2 plays a crucial role in the immune response by inducing the infiltration of neutrophils to sites of infection or injury . This chemotactic activity is essential for the body’s defense against pathogens. CXCL2 is produced by macrophages and monocytes and helps with the chemotaxis of polymorphonuclear leukocytes and hematopoietic stem cells . It also suppresses the proliferation of hematopoietic progenitor cells .
Recombinant CXCL2 is widely used in research, particularly in studies related to inflammation and oncology . It is used in cell culture applications to study the behavior of neutrophils and other immune cells in response to chemotactic signals. The protein’s ability to induce chemotaxis makes it a valuable tool for understanding the mechanisms of immune cell migration and the body’s response to infection and injury.
The lyophilized form of human GRO-Beta should be stored at 2-8°C, preferably desiccated . Once reconstituted, it should be stored at -20°C to -70°C to maintain its stability and biological activity . It is important to avoid repeated freeze-thaw cycles, as this can degrade the protein and reduce its effectiveness.
