GRO b Human
GRO-Beta Human Recombinant (CXCL2)
Cat. No.
BT12936
Source
Escherichia Coli.
Synonyms
Macrophage inflammatory protein 2-alpha, MIP2-alpha, CXCL2, Growth- regulated protein beta, Gro-beta, chemokine (C-X-C motif) ligand 2, GRO2, GROb, MIP2, MIP2A, SCYB2, MGSA-b, MIP-2a, CINC-2a, MGSA beta.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
GRO-Beta Human Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7908 Dalton.
The CXCL2 is purified by proprietary chromatographic techniques.
The CXCL2 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Chemokine (C-X-C motif) ligand 2 (CXCL2), also known as macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta), and Gro oncogene-2 (Gro-2), is a small cytokine of the CXC chemokine family. Sharing a 90% amino acid sequence identity with chemokine CXCL1, CXCL2 is secreted by monocytes and macrophages, and exhibits chemotactic properties for polymorphonuclear leukocytes and hematopoietic stem cells. The gene encoding CXCL2 is found on human chromosome 4 within a cluster of other CXC chemokines. CXCL2 interacts with the cell surface chemokine receptor CXCR2 to mobilize cells.
Description
Recombinant Human GRO-Beta, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 73 amino acids. With a molecular weight of 7.9 kDa, CXCL2 undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Sterile filtered white lyophilized powder.
Formulation
The protein was lyophilized without any additional additives.
Solubility
For reconstitution of lyophilized Human GRO-beta, sterile 18MΩ-cm H2O is recommended at a concentration not less than 100 µg/ml. Further dilutions can be made using other aqueous solutions.
Stability
Lyophilized CXCL2 remains stable at room temperature for up to 3 weeks. However, it is recommended to store it desiccated below -18°C. After reconstitution, store CXCL2 at 4°C for 2-7 days. For long-term storage, store below -18°C. It is advisable to add a carrier protein (0.1% HSA or BSA) for long-term storage and to avoid freeze-thaw cycles.
Purity
Purity exceeds 98.0% as determined by: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
Biological activity is assessed through the chemoattraction of CXCR2 transfected 293 cells using a concentration range of 10-100 ng/ml, corresponding to a specific activity of 10,000-100,000 IU/mg.
Synonyms
Macrophage inflammatory protein 2-alpha, MIP2-alpha, CXCL2, Growth- regulated protein beta, Gro-beta, chemokine (C-X-C motif) ligand 2, GRO2, GROb, MIP2, MIP2A, SCYB2, MGSA-b, MIP-2a, CINC-2a, MGSA beta.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Leu-Ala-Thr.
Product Science Overview
Gene and Protein Structure
The human GRO-Beta is encoded by the CXCL2 gene. This gene is one of three highly related chemokines, the others being GRO-Alpha (CXCL1) and GRO-Gamma (CXCL3). These chemokines share significant sequence homology and bind to the same receptors, albeit with differing affinities . The recombinant human GRO-Beta is a non-glycosylated protein consisting of 73 amino acids and has a molecular mass of approximately 7.9 kDa .
Biological Functions
GRO-Beta is produced by activated monocytes and neutrophils and is expressed at sites of inflammation . It is known for its role in:
- Chemotaxis: GRO-Beta attracts neutrophils to sites of infection or injury, aiding in the immune response .
- Angiogenesis: It promotes the formation of new blood vessels, which is essential for wound healing and tissue repair .
- Growth Regulation: GRO-Beta influences the growth and proliferation of various cell types .
Recombinant Production
Recombinant human GRO-Beta is typically produced in E. coli. The protein is often lyophilized (freeze-dried) and can be reconstituted in sterile water or phosphate-buffered saline (PBS) for use in various research applications . The recombinant form is used extensively in research to study its biological activities and potential therapeutic applications.
Applications in Research
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