GRO a Human, His

GRO-Alpha/MGSA Human Recombinant (CXCL1), His Tag
Cat. No.
BT12777
Source
Escherichia Coli.
Synonyms
Growth-regulated protein alpha, CXCL1, Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3, GRO-alpha(1-73), chemokine (C-X-C motif) ligand 1, GRO1, GROa, SCYB1, MGSA-a, MGSA alpha.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CXCL1 Human Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 94 amino acids (35-107) and having a molecular mass of 10.1 kDa. The CXCL1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Growth-regulated oncogene alpha (GROα), also known as chemokine (C-X-C motif) ligand 1 (CXCL1), is a small cytokine belonging to the CXC chemokine family. It was previously referred to as GRO1 oncogene, Neutrophil-activating protein 3 (NAP-3), and melanoma growth stimulating activity, alpha (MSGA-α). CXCL1 is secreted by various cells, including macrophages, neutrophils, and epithelial cells. This chemokine plays a crucial role in neutrophil chemotaxis and has been implicated in several physiological processes such as angiogenesis, inflammation, wound healing, and tumorigenesis. CXCL1 exerts its biological effects by binding to the chemokine receptor CXCR2. The gene encoding CXCL1 is located on human chromosome 4, clustered with genes encoding other CXC chemokines.
Description
Recombinant human CXCL1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It encompasses amino acids 35-107, resulting in a protein with a molecular weight of 10.1 kDa. For purification purposes, a 20 amino acid His-Tag is fused to the N-terminus of the protein. The purification process employs proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The formulation consists of CXCL1 dissolved in a buffer solution containing 20mM Tris-HCL at pH 8.0 and 10% glycerol.
Stability
CXCL1 in liquid form is stable for up to one week when stored at 10°C. However, for long-term storage, it is recommended to store the protein below -18°C. To enhance stability during long-term storage, the addition of a carrier protein like HSA or BSA (0.1%) is advisable. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the CXCL1 protein is greater than 90.0%, as determined by SDS-PAGE analysis.
Synonyms
Growth-regulated protein alpha, CXCL1, Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3, GRO-alpha(1-73), chemokine (C-X-C motif) ligand 1, GRO1, GROa, SCYB1, MGSA-a, MGSA alpha.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASVATELRC QCLQTLQGIH PKNIQSVNVK SPGPHCAQTE VIATLKNGRK ACLNPASPIV KKIIEKMLNS DKSN.

Product Science Overview

Introduction

GRO-Alpha, also known as Growth-Regulated Oncogene Alpha (GRO-Alpha) or Melanoma Growth Stimulatory Activity Alpha (MGSA), is a chemokine belonging to the CXC chemokine family. It is encoded by the CXCL1 gene and plays a crucial role in various physiological and pathological processes, including inflammation, wound healing, and cancer progression.

Structure and Characteristics

GRO-Alpha is a small cytokine with a molecular weight of approximately 7.8 kDa . It consists of 73 amino acids and includes the ‘ELR’ motif, which is characteristic of the CXC chemokine family . The recombinant form of GRO-Alpha, often tagged with a His (histidine) tag for purification purposes, is produced in Escherichia coli (E. coli) expression systems .

Biological Functions

GRO-Alpha is primarily known for its role in the recruitment and activation of neutrophils, a type of white blood cell essential for the immune response . It binds with high affinity to the IL-8 receptor type B (CXCR2), leading to the activation and chemotaxis of neutrophils to sites of tissue injury or infection . This chemokine is also involved in angiogenesis, the formation of new blood vessels, which is critical for wound healing and tumor growth .

Clinical Significance

GRO-Alpha has been implicated in various diseases, including cancer, inflammatory conditions, and autoimmune disorders. Its overexpression has been observed in several types of cancer, such as melanoma, where it promotes tumor growth and metastasis . Additionally, GRO-Alpha is involved in the pathogenesis of inflammatory diseases like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD) .

Applications in Research

Recombinant GRO-Alpha is widely used in research to study its biological functions and therapeutic potential. It is utilized in various assays, including chemotaxis assays, to investigate its role in immune cell recruitment and activation . The His tag facilitates the purification and detection of the recombinant protein, making it a valuable tool for biochemical and structural studies .

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