MIP2 Viral

Viral MIP-2 is closely related to MIP-1α, sharing about 41% amino acid sequence similarity . At the amino acid sequence level, Viral MIP-1 and Viral MIP-2 share 48% similarity . These chemokines are more closely linked to one another phylogenetically than to other human chemokines, indicating that they likely arose from gene duplication within the virus rather than from two independent gene acquisitions .

The recombinant form of Viral MIP-2 is typically produced in Escherichia coli and is a non-glycosylated polypeptide chain containing 70 amino acids with a molecular mass of approximately 7.9 kDa . The protein is purified using proprietary chromatographic techniques to ensure high purity and biological activity .

Viral MIP-2 binds to the CCR3 chemokine receptor, through which it activates and chemoattracts human eosinophils . This interaction is crucial for the immune response, as it helps direct immune cells to sites of infection or inflammation. The biological activity of Viral MIP-2 is often assessed by its ability to inhibit monocyte migration in response to human MIP-1α .

The recombinant form of Viral MIP-2 is used in various research applications, including functional studies, HPLC, and SDS-PAGE . It is particularly valuable for studying the mechanisms of chemokine-receptor interactions and the role of chemokines in immune responses. Researchers also use Viral MIP-2 to explore potential therapeutic applications, such as developing treatments for inflammatory diseases and understanding viral pathogenesis.

Lyophilized Viral MIP-2 protein is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, the protein should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use . To prevent freeze-thaw cycles, it is recommended to add a carrier protein such as 0.1% HSA or BSA .