GRO a Rat

GRO-Alpha Rat Recombinant (CXCL1)
Cat. No.
BT12871
Source
Escherichia Coli.
Synonyms
Growth-regulated protein alpha, CXCL1, Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3, GRO-alpha(1-73), chemokine (C-X-C motif) ligand 1, GRO1, GROa, SCYB1, MGSA-a, MGSA alpha.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

CXCL1 Rat Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7.8 kDa.
The Rat CXCL1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Chemokine (C-X-C motif) ligand 1 (CXCL1), also known as GRO1 oncogene, Neutrophil-activating protein 3 (NAP-3), and melanoma growth stimulating activity, alpha (MSGA-α), is a small cytokine in the CXC chemokine family. It is secreted by human melanoma cells and has mitogenic properties, playing a role in melanoma pathogenesis. CXCL1 is produced by macrophages, neutrophils, and epithelial cells and acts as a neutrophil chemoattractant. It is involved in spinal cord development, where it inhibits the migration of oligodendrocyte precursors. Additionally, CXCL1 contributes to angiogenesis, inflammation, wound healing, and tumorigenesis. Its effects are mediated by signaling through the chemokine receptor CXCR2. The gene encoding CXCL1 is found on human chromosome 4, clustered with genes for other CXC chemokines.
Description
Recombinant Rat CXCL1, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 73 amino acids. With a molecular weight of 7.8 kDa, Rat CXCL1 is purified through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 0.2 μm filtered solution at a concentration of 1 mg/ml in 20 mM phosphate buffer (PB), pH 7.4, with 150 mM NaCl.
Solubility
Reconstitute the lyophilized CXCL1 in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized CXCL1 remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store desiccated below -18°C. Reconstituted CXCL1 should be stored at 4°C for 2-7 days. For long-term storage, add a carrier protein (0.1% HSA or BSA) and store below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0% as determined by: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The biological activity of Rat CXCL1 is evaluated based on its ability to chemoattract rat neutrophils. Using a concentration range of 10-100 ng/ml, the specific activity corresponds to 10,000-100,000 IU/mg.
Synonyms
Growth-regulated protein alpha, CXCL1, Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3, GRO-alpha(1-73), chemokine (C-X-C motif) ligand 1, GRO1, GROa, SCYB1, MGSA-a, MGSA alpha.
Source
Escherichia Coli.
Amino Acid Sequence
APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK.

Product Science Overview

Structure and Function

CXCL1 is characterized by the presence of a conserved glutamic acid-leucine-arginine (ELR) motif. This motif is crucial for its function, as it allows CXCL1 to bind to its receptor, CXCR2 . The binding of CXCL1 to CXCR2 triggers a series of intracellular signaling pathways that lead to the migration and activation of neutrophils .

Biological Role

CXCL1 plays a significant role in the inflammatory response. It is produced by various cell types, including macrophages, epithelial cells, and fibroblasts, in response to inflammatory stimuli . Once released, CXCL1 acts as a chemoattractant, guiding neutrophils to the site of infection or injury. This process is essential for the clearance of pathogens and the initiation of tissue repair .

Clinical Significance

High levels of CXCL1 have been associated with various pathological conditions, including cancer and chronic inflammatory diseases . For instance, elevated CXCL1 expression has been linked to advanced stages of uterine cervical cancer and worse survival outcomes . In cancer, CXCL1 can promote tumor growth and metastasis by enhancing the migration and invasion of cancer cells .

Research Applications

The rat recombinant version of CXCL1 is widely used in research to study its role in different biological processes and disease models. It is particularly useful in experiments involving neutrophil migration and activation, as well as in studies exploring the molecular mechanisms underlying inflammation and cancer progression .

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