GRO1/KC Mouse, His

GRO/KC (CXCL1) Mouse Recombinant, His Tag
Cat. No.
BT14420
Source
Escherichia Coli.
Synonyms
Growth-regulated alpha protein, CXCL1, Platelet-derived growth factor-inducible protein KC, Secretory protein N51, KC, Fsp, N51, gro, Gro1, Mgsa, Scyb1, chemokine (C-X-C motif) ligand 1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GRO1/KC Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids (25-96 a.a.) and having a molecular mass of 10.5kDa.
GRO1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Chemokine (C-X-C motif) ligand 1 (CXCL1), formerly known as GRO1 oncogene, Neutrophil-activating protein 3 (NAP-3), and melanoma growth stimulating activity, alpha (MSGA-a), is a small cytokine of the CXC chemokine family. Produced by human melanoma cells, CXCL1 exhibits mitogenic properties and plays a role in melanoma development. This chemokine is also expressed by macrophages, neutrophils, and epithelial cells, demonstrating neutrophil chemoattractant activity. CXCL1 is involved in spinal cord development by suppressing the migration of oligodendrocyte precursors and participates in angiogenesis, inflammation, wound healing, and tumorigenesis. It exerts its effects by signaling through the CXCR2 chemokine receptor. The gene encoding CXCL1 is located on human chromosome 4, clustered with genes for other CXC chemokines.
Description
Recombinant Mouse GRO1/KC, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 97 amino acids (25-96 a.a.), has a molecular weight of 10.5 kDa, and includes a 25 amino acid His-tag at the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The GRO1 protein solution is provided at a concentration of 0.5 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 0.1 M NaCl.
Stability
For short-term storage (up to 4 weeks), store the vial at 4°C. For extended storage, freeze the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
Growth-regulated alpha protein, CXCL1, Platelet-derived growth factor-inducible protein KC, Secretory protein N51, KC, Fsp, N51, gro, Gro1, Mgsa, Scyb1, chemokine (C-X-C motif) ligand 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAPIAN ELRCQCLQTM AGIHLKNIQS LKVLPSGPHC TQTEVIATLK NGREACLDPE APLVQKIVQK MLKGVPK.

Product Science Overview

Discovery and Nomenclature

CXCL1 was first cloned from a cDNA library of genes induced by platelet-derived growth factor (PDGF) stimulation of BALB/c-3T3 murine embryonic fibroblasts. It was named “KC” for its location in the nitrocellulose colony hybridization assay . Over time, it has been referred to by various names, including GRO-α and GRO1 oncogene .

Structure and Function

The recombinant mouse CXCL1/KC protein is typically produced in E. coli and is a single, non-glycosylated polypeptide chain containing 97 amino acids (25-96) with a molecular weight of approximately 8 kDa . The His Tag is often added to facilitate purification and detection of the protein.

CXCL1 is a potent chemoattractant for neutrophils, playing a significant role in inflammatory responses. It binds to the CXCR2 receptor on the surface of neutrophils, leading to their migration to sites of infection or injury. This chemokine is also involved in angiogenesis, wound healing, and tumorigenesis .

Applications in Research

Recombinant mouse CXCL1/KC with a His Tag is widely used in research to study its role in various biological processes, including inflammation, cancer, and tissue repair. It is also used to investigate the signaling pathways involved in neutrophil recruitment and activation .

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