GRO g Mouse
GRO-Gamma Mouse Recombinant (CXCL3)
Cat. No.
BT14044
Source
Escherichia Coli.
Synonyms
C-X-C motif chemokine 3, Dendritic cell inflammatory protein 1, Cxcl3, Dcip1, Gm1960.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
GRO-g Mouse Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7.7kDa.
The CXCL3 is purified by proprietary chromatographic techniques.
The CXCL3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine that belongs to the CXC chemokine family. It is also known as GRO3 oncogene (GRO3), GRO protein gamma (GROg), and macrophage inflammatory protein-2-beta (MIP2b). CXCL3 regulates the migration and adhesion of monocytes. It exerts its effects on target cells by interacting with a cell surface chemokine receptor called CXCR2. The gene encoding CXCL3 is found on chromosome 4, clustered with other CXC chemokines.
Description
Recombinant Mouse GRO-g, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 69 amino acids. It has a molecular weight of 7.7 kDa. The purification of CXCL3 is carried out using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The Mouse CXCL3 is lyophilized from a 0.2 µm filtered solution in 1x PBS, pH 7.4.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized GRO-gamma in sterile 18 MΩ·cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized GRO-gamma remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. Once reconstituted, CXCL3 should be stored at 4°C for 2-7 days. For long-term storage, it is advisable to store it below -18°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 97.0% by the following methods:
(a) RP-HPLC analysis
(b) SDS-PAGE analysis
Biological Activity
The biological activity is evaluated based on the ability to induce chemotaxis in CXCR2-transfected 293 cells. This is assessed within a concentration range of 10-100 ng/ml, corresponding to a specific activity of 10,000-100,000 units/mg.
Synonyms
C-X-C motif chemokine 3, Dendritic cell inflammatory protein 1, Cxcl3, Dcip1, Gm1960.
Source
Escherichia Coli.
Amino Acid Sequence
SELRCQCLNT LPRVDFETIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QGPRLQIIIK KILKSGKSS.
Product Science Overview
Gene and Protein Structure
The gene encoding CXCL3 in mice is referred to as Cxcl3. The recombinant mouse CXCL3 protein typically consists of 83 amino acids and has a predicted molecular mass of approximately 9.3 kDa . The protein is often expressed with a polyhistidine tag at the C-terminus to facilitate purification and detection .
Biological Functions
CXCL3 is involved in several key biological processes:
- Neutrophil Attraction and Activation: Similar to other GRO proteins, CXCL3 is a potent attractant and activator of neutrophils .
- Trophoblast Migration and Invasion: CXCL3 plays a role in the migration, invasion, proliferation, and tubule formation of trophoblasts, which are essential for placental development .
- Cancer Progression: CXCL3 and its receptor CXCR2 are overexpressed in prostate cancer cells and tissues, suggesting a role in cancer progression and metastasis .
- Adipogenesis: CXCL3 acts as a novel adipokine, facilitating adipogenesis through the induction of specific transcription factors .
Expression and Regulation
CXCL3 is expressed in various tissues and cells, including immune cells, epithelial cells, and cancer cells. Its expression is regulated by several factors, including inflammatory cytokines and growth factors.
Recombinant Production
Applications
Recombinant CXCL3 is used in various research applications, including:
- Cell Migration Assays: To study the chemotactic properties of CXCL3.
- Cancer Research: To investigate the role of CXCL3 in cancer progression and metastasis.
- Immunology Studies: To explore the function of CXCL3 in immune responses.
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