GRO g Human

Human GRO-Gamma is a small protein with a molecular weight of approximately 7.9 kDa and consists of 73 amino acid residues . It is produced as a precursor protein that undergoes cleavage to generate the mature form. The functional receptor for CXCL3 is CXCR2, which is a G-protein-coupled receptor .

CXCL3 plays a significant role in the immune system by acting as a potent chemoattractant and activator of neutrophils. Neutrophils are a type of white blood cell essential for the body’s defense against infections. CXCL3 promotes the chemotaxis (movement) and degranulation (release of granules) of neutrophils and basophils, which are other types of white blood cells .

Recombinant human CXCL3 is typically produced in Escherichia coli (E. coli) expression systems. The recombinant protein is purified to high levels of purity, often exceeding 95% as determined by SDS-PAGE with silver staining . The endotoxin levels are kept very low to ensure the protein’s safety and efficacy in research and therapeutic applications .

Recombinant CXCL3 is widely used in research to study its role in immune responses and inflammation. It is also utilized in various assays to investigate its chemoattractant properties and its ability to induce the release of myeloperoxidase from neutrophils . Additionally, CXCL3 is used in cell culture studies to understand its effects on different cell types and its potential therapeutic applications.

Recombinant CXCL3 is typically lyophilized and can be reconstituted in sterile phosphate-buffered saline (PBS) for use. It is recommended to store the protein at -20 to -70 °C to maintain its stability and avoid repeated freeze-thaw cycles .