GRO g/CINC-2a Rat

GRO-Gamma Rat Recombinant (CXCL3), CINC-2 alpha
Cat. No.
BT14259
Source
Escherichia Coli.
Synonyms
Cytokine-induced neutrophil chemoattractant 2, CINC2, CINC-2, Macrophage inflammatory protein 2-alpha.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GRO-g Rat Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7.8kDa. The CXCL3 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Chemokine (C-X-C motif) ligand 3 (CXCL3), alternatively known as GRO3 oncogene (GRO3), GRO protein gamma (GROg), or macrophage inflammatory protein-2-beta (MIP2b), is a small cytokine belonging to the CXC chemokine family. This chemokine plays a crucial role in regulating the migration and adhesion of monocytes. CXCL3 exerts its effects by binding to a cell surface chemokine receptor known as CXCR2. The gene encoding CXCL3 is located on chromosome 4, clustered with other CXC chemokines.
Description
Recombinant Rat GRO-g, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 69 amino acids. With a molecular weight of 7.8 kDa, CXCL3 undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The Rat CXCL3 is lyophilized from a 0.2 µm filtered concentrated solution in 1xPBS, pH 7.4.
Solubility
To reconstitute the lyophilized GRO-gamma, it is recommended to dissolve it in sterile 18 MΩ cm H2O at a concentration not less than 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized GRO-gamma demonstrates stability at room temperature for a period of 3 weeks. However, for extended storage, it is advisable to store it in a desiccated state below -18°C. Upon reconstitution, CXCL3 should be stored at 4°C for a period of 2-7 days. For long-term storage, it is recommended to freeze it below -18°C. To ensure optimal preservation, it is crucial to avoid repeated freeze-thaw cycles. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Purity
The purity of the product exceeds 98.0%, as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity
The biological activity is determined by the ability to induce chemotaxis of 293 cells transfected with CXCR2. This is assessed within a concentration range of 10-100 ng/ml, corresponding to a specific activity of 10,000-100,000 units/mg.
Synonyms
Cytokine-induced neutrophil chemoattractant 2, CINC2, CINC-2, Macrophage inflammatory protein 2-alpha.
Source
Escherichia Coli.
Amino Acid Sequence
RELRCQCLKT LPRVDFENIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QAPRLQKIIQ KLLKSDKSS.

Product Science Overview

Structure and Function

GRO-Gamma is a non-glycosylated polypeptide chain consisting of 68 amino acids. It is produced in E. coli and purified through high-performance liquid chromatography (HPLC). The protein has a molecular weight of approximately 7.6 kDa . The functional receptor for CXCL3 is CXCR2, which is involved in the chemotaxis of neutrophils .

Biological Activity

The biological activity of GRO-Gamma is determined by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils and to chemoattract BaF3 mouse pro-B cells transfected with human CXCR2. The effective dose (ED50) for these activities ranges from 3-6 µg/mL and 4-20 ng/mL, respectively .

Preparation and Storage

Recombinant GRO-Gamma is typically lyophilized from a 0.2 μm filtered solution in acetonitrile and trifluoroacetic acid (TFA). It can be reconstituted in sterile phosphate-buffered saline (PBS) containing at least 0.1% human or bovine serum albumin. The protein is stable for 12 months when stored at -20 to -70°C and for 1 month at 2 to 8°C under sterile conditions after reconstitution .

Applications

GRO-Gamma is used in various research applications, including studies on neutrophil chemotaxis, inflammation, and immune responses. It is also utilized in bioassays to test the activity of CXCR2 and other related receptors .

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