GRO-g/CINC-2b Rat
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
GRO-g is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
GRO-gamma, also known as CINC-2 beta (Rat Recombinant CXCL3), is a member of the CXC chemokine family. This family of chemokines is known for its role in the immune response, particularly in the recruitment and activation of neutrophils. The rat chemokines CINC (cytokine-induced neutrophil chemoattractant)-1, CINC-2 alpha, CINC-2 beta, and CINC-3 (also known as MIP-2) are homologous to human GROs and mouse MIP-2 . These chemokines are crucial in mediating inflammatory responses and are involved in various physiological and pathological processes.
The recombinant rat CXCL3/CINC-2 beta protein is typically produced using Escherichia coli (E. coli) as the expression system. The gene encoding the CXCL3 protein is cloned into an expression vector, which is then introduced into E. coli cells. The bacteria are cultured, and the protein is expressed and accumulated within the cells. The cells are then lysed to release the protein, which is subsequently purified using techniques such as high-performance liquid chromatography (HPLC) and SDS-PAGE .
The purified protein is lyophilized from a filtered solution, often containing acetonitrile and trifluoroacetic acid (TFA), to ensure stability and ease of storage. The lyophilized protein can be reconstituted in sterile phosphate-buffered saline (PBS) for use in various applications .
The biological activity of recombinant rat CXCL3/CINC-2 beta is determined through various assays. One common method is the chemotaxis bioassay, which measures the protein’s ability to attract neutrophils or other cell types expressing the CXCR2 receptor. The effective dose (ED50) for this activity is typically in the range of 5-25 ng/mL .
The protein’s structure and purity are analyzed using SDS-PAGE and HPLC. The amino acid sequence of CXCL3/CINC-2 beta is confirmed through sequencing, ensuring that the recombinant protein matches the native sequence. The protein is a single non-glycosylated polypeptide chain containing 69 amino acids, with a molecular weight of approximately 7.8 kDa .
