GPT Rat

The recombinant form of GPT from rats is a bioactive protein with a molecular weight of approximately 55 kDa . It is typically produced in E. coli and includes a His-tag for purification purposes . The enzyme consists of 496 amino acids and is found in various tissues, including the liver, kidney, heart, and skeletal muscles .

GPT’s primary function is to facilitate the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate . This reaction is essential for the metabolism of nitrogen and the production of glucose in the liver. The enzyme’s activity is crucial for maintaining the balance of amino acids and energy production in cells .

GPT is widely used as a biomarker for liver function. Elevated levels of GPT in the blood can indicate liver damage or disease, such as hepatitis, cirrhosis, or liver cancer . It is also used in research to study various diseases and disorders, including liver disease, glucose intolerance, and viral hepatitis .

Recombinant GPT is used in various research applications, including enzyme activity assays, protein-protein interaction studies, and drug development . Its high purity and specific activity make it a valuable tool for studying the biochemical pathways involving amino acid metabolism and liver function .

Recombinant GPT should be stored at 4°C for short-term use and at -20°C for long-term storage to maintain its stability and activity . It is typically supplied in a buffer containing PBS (pH 7.4) and 10% glycerol to prevent degradation .