GPT Mouse

GPT belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family and has two distinct molecular and genetic forms: one cytoplasmic (soluble) known as GPT1 and one mitochondrial known as GPT2 . The enzyme is a single, non-glycosylated polypeptide chain containing 519 amino acids and has a molecular mass of approximately 57.5 kDa .

GPT is widely used as an indicator of liver health or hepatocellular damage in clinical tests . Its activity is measured to assess liver function, as elevated levels of GPT in the blood can indicate liver damage or disease. The enzyme’s role in converting alanine and alpha-ketoglutarate to glutamate and pyruvate is essential for the metabolism of amino acids and glucose .

The recombinant form of GPT from mice is produced in Escherichia coli (E. coli). This recombinant protein is often used in research and clinical applications due to its high purity and specific activity . The production process involves the expression of the GPT gene in E. coli, followed by purification using proprietary chromatographic techniques .

Recombinant GPT is used in various research applications, including studies on liver function, metabolism, and enzyme kinetics. It is also employed in the development of diagnostic assays for liver diseases .

The recombinant GPT protein is typically stored at 4°C for short-term use and at -20°C for long-term storage. It is recommended to avoid multiple freeze-thaw cycles to maintain the protein’s stability and activity .