GPT Human, His Active

Glutamic-Pyruvate Transaminase, His Tag Active Human Recombinant
Cat. No.
BT1387
Source
E.coli.
Synonyms
Glutamic-pyruvate transaminase (alanine aminotransferase), GPT1, ALT1, AAT1, Glutamic-alanine transaminase 1, EC 2.6.1.2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GPT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 516 amino acids (1-496) and having a molecular mass of 56.8 kDa.
GPT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
GPT (Glutamic-pyruvic transaminase) is an enzyme that plays a vital role in the metabolism of amino acids and glucose. It catalyzes the reversible conversion of alanine and 2-oxoglutarate into pyruvate and glutamate. GPT is a key indicator of liver health and is frequently used in clinical settings to assess liver function and detect potential damage.
Description
This product consists of recombinant human GPT, expressed in E. coli and purified to a high degree. It is a single polypeptide chain, lacking glycosylation, with 516 amino acids (residues 1-496). The protein has a molecular weight of 56.8 kDa. For purification and detection purposes, a 20 amino acid His-tag is fused to the N-terminus.
Physical Appearance
The product is a sterile, colorless solution.
Formulation
The GPT enzyme is supplied in a solution at a concentration of 0.5mg/ml. The solution also contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT (dithiothreitol), and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of GPT Human Recombinant is determined by SDS-PAGE analysis and is guaranteed to be greater than 90%.
Biological Activity
The specific activity of GPT is measured as the amount of enzyme required to convert 1 micromole of L-Alanine to L-Glutamate per minute at a pH of 7.5 and a temperature of 37°C. The specific activity is greater than 100 units per milligram of enzyme.
Synonyms
Glutamic-pyruvate transaminase (alanine aminotransferase), GPT1, ALT1, AAT1, Glutamic-alanine transaminase 1, EC 2.6.1.2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS

Product Science Overview

Introduction

Glutamic-Pyruvate Transaminase (GPT), also known as Alanine Aminotransferase (ALT), is an enzyme that plays a crucial role in the metabolism of amino acids and glucose. It catalyzes the reversible transamination between L-alanine and alpha-ketoglutarate to produce L-glutamate and pyruvate . This enzyme is found in various tissues, but it is most abundant in the liver .

Structure and Function

GPT belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family and has two distinct molecular and genetic forms: one cytoplasmic (GPT1) and one mitochondrial (GPT2) . The enzyme requires the coenzyme pyridoxal phosphate for its activity . The His Tag Active form of GPT is a recombinant version that includes a histidine tag, which facilitates its purification and detection in research applications .

Clinical Significance

GPT is widely used as a biomarker for liver health. Elevated levels of GPT in the blood can indicate liver damage or disease, such as viral hepatitis, diabetes, or bile duct problems . It is commonly measured in liver function tests alongside aspartate aminotransferase (AST), and the ratio of AST to ALT is used to diagnose various liver conditions .

Recombinant GPT

The recombinant form of GPT, particularly the His Tag Active version, is produced using advanced biotechnological methods. This involves the expression of the human GPT gene in a suitable host system, followed by purification using the histidine tag . The recombinant enzyme retains the functional properties of the native enzyme and is used in various research and diagnostic applications .

Applications

Recombinant GPT is used in:

  • Biochemical Research: Studying the enzyme’s role in amino acid and glucose metabolism.
  • Clinical Diagnostics: Developing assays for liver function tests.
  • Drug Development: Screening for potential hepatotoxicity of new drugs.

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