GPT Human, Active

The human recombinant form of GPT is produced in E. coli and is a homodimer, non-glycosylated polypeptide chain containing 495 amino acids with a molecular mass of approximately 54,479 Daltons . The amino acid sequence of this recombinant enzyme is identical to that of the native form found in the human liver .

GPT is involved in cellular nitrogen metabolism and liver gluconeogenesis, starting with precursors transported from skeletal muscles . It is widely used as a biomarker for liver health, as elevated levels of GPT in the serum can indicate liver injury caused by drug toxicity, infection, alcohol, and steatosis .

The specific activity of the recombinant GPT enzyme is found to be 1,020 U/mg . It is used in various clinical tests to assess liver function and diagnose liver diseases. The enzyme’s activity levels in the serum are routinely measured to monitor liver health and detect potential liver damage .

The recombinant GPT enzyme is stable at 10°C for up to 5 days but should be stored below -18°C to prevent freeze-thaw cycles, which can affect its stability . The enzyme is typically formulated in a sterile liquid solution containing sodium acetate buffer, DTT, EDTA, 2-oxoglutarate, and pyridoxal-5’-phosphate .