GOT2 Mouse

Glutamic-Oxaloacetic Transaminase 2 Mouse Recombinant
Cat. No.
BT1076
Source
Escherichia Coli.
Synonyms

Transaminase A, KAT4, KATIV, KAT-4, KAT-IV,Kynurenine Aminotransferase 4.

Appearance
Sterile filtered colorless solution.
Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GOT2 Recombinant Mouse produced in E.Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (30-430 a.a.) and having a molecular mass of 46.8kDa. Mouse GOT2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
GOT2, also known as mitochondrial aspartate aminotransferase (mAST), is a pyridoxal phosphate-dependent enzyme that plays a crucial role in amino acid metabolism and the urea and tricarboxylic acid cycles. GOT2 is localized in the mitochondria, while its cytosolic counterpart, GOT1, is found in the cytoplasm. Both GOT1 and GOT2 are homodimeric enzymes and share significant sequence homology.
Description
Recombinant Mouse GOT2, expressed in E. coli, is a monomeric, non-glycosylated polypeptide chain with a molecular weight of 46.8 kDa. The recombinant protein encompasses amino acids 30 to 430 of the GOT2 sequence, fused to a 21 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Sterile filtered, colorless solution.
Formulation
The GOT2 solution is provided at a concentration of 0.5 mg/ml in a buffer containing PBS (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the GOT2 solution should be kept at 4°C. For long-term storage, it is recommended to store the solution at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is recommended for long-term storage to enhance protein stability. Avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity
The purity of the GOT2 protein is greater than 90%, as determined by SDS-PAGE analysis.
Biological Activity
The enzyme activity of GOT2 is greater than 20 units/mg. One unit of activity is defined as the amount of enzyme required to catalyze the conversion of 1 μmole of alpha-ketoglutarate to L-Glutamate per minute at a pH of 8.0 and a temperature of 25°C.
Synonyms

Transaminase A, KAT4, KATIV, KAT-4, KAT-IV,Kynurenine Aminotransferase 4.

Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSSWWTHVEM GPPDPILGVT EAFKRDTNSK KMNLGVGAYR DDNGKPYVLP SVRKAEAQIA AKNLDKEYLP IGGLAEFCKA SAELALGENN EVLKSGRFVT VQTISGTGAL RVGASFLQRF FKFSRDVFLP KPSWGNHTPI FRDAGMQLQG YRYYDPKTCG FDFSGALEDI SKIPEQSVLL LHACAHNPTG VDPRPEQWKE IASVVKKKNL FAFFDMAYQG FASGDGDKDA WAVRHFIEQG INVCLCQSYA KNMGLYGERV GAFTVVCKDA EEAKRVESQL KILIRPLYSN PPLNGARIAA TILTSPDLRK QWLQEVKGMA DRIISMRTQL VSNLKKEGSS HNWQHITDQI GMFCFTGLKP EQVERLTKEF SVYMTKDGRI SVAGVTSGNV GYLAHAIHQV.

Product Science Overview

Gene and Protein Structure

The GOT2 gene encodes the mitochondrial form of the enzyme and is highly conserved across species . The enzyme is homodimeric, meaning it consists of two identical subunits . The gene is located on chromosome 16 in humans and has several aliases, including mitochondrial aspartate aminotransferase (mAspAT) and kynurenine aminotransferase IV (KYAT4) .

Function and Mechanism

GOT2 catalyzes the reversible transamination between glutamate and oxaloacetate to form aspartate and alpha-ketoglutarate . This reaction is a key step in the malate-aspartate shuttle, which is essential for transferring reducing equivalents across the mitochondrial membrane . The enzyme also plays a role in the kynurenine pathway, converting L-kynurenine to kynurenic acid .

Biological Significance

GOT2 is involved in several critical metabolic pathways:

  • Amino Acid Metabolism: It facilitates the interconversion of amino acids and alpha-keto acids, which is vital for protein synthesis and degradation .
  • Urea Cycle: The enzyme helps in the detoxification of ammonia by converting it into urea for excretion .
  • Krebs Cycle: It contributes to the production of energy by participating in the Krebs cycle .
Clinical Relevance

Mutations in the GOT2 gene have been associated with various diseases, including Developmental and Epileptic Encephalopathy 82 and Pheochromocytoma . The enzyme’s activity is also a marker for liver function tests, as elevated levels can indicate liver damage .

Research and Applications

Recombinant mouse GOT2 is widely used in research to study its function and role in various metabolic pathways. It is also used to develop assays for measuring enzyme activity and to screen for potential inhibitors that could be used in therapeutic applications .

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<p><span lang="EN-US">Escherichia Coli.</span></p>
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