GOT2 Human

GOT2 is a homodimeric enzyme, meaning it consists of two identical subunits. Each subunit has several distinct regions, including a large domain that binds pyridoxal phosphate, a small domain, an NH2-terminal arm, and a bridge across two domains . The enzyme catalyzes the reversible transamination of aspartate and alpha-ketoglutarate to form oxaloacetate and glutamate .

One of the key roles of GOT2 is in the malate-aspartate shuttle, which is essential for transferring reducing equivalents across the mitochondrial membrane. This shuttle is crucial for maintaining the NAD+/NADH balance within cells, which is vital for energy production .

GOT2 is involved in several metabolic pathways, including the urea cycle and the tricarboxylic acid cycle (also known as the Krebs cycle) . It also plays a significant role in the metabolism of amino acids and the production of energy within cells .

In addition to its metabolic functions, GOT2 has been implicated in cell proliferation and tumor growth. Studies have shown that GOT2 activity is upregulated in certain types of cancer, suggesting that it may play a role in supporting the increased metabolic demands of rapidly dividing tumor cells .

Recombinant human GOT2 is widely used in research to study its role in metabolism and disease. It is produced using recombinant DNA technology, which involves inserting the human GOT2 gene into a suitable expression system, such as bacteria or yeast, to produce the enzyme in large quantities .

Researchers use recombinant GOT2 to investigate its structure, function, and interactions with other molecules. This research can provide insights into the enzyme’s role in health and disease and may lead to the development of new therapeutic strategies for conditions such as cancer and metabolic disorders .