GOT2 Mouse, Active
Escherichia Coli.
Transaminase A, KAT4, KATIV, KAT-4, KAT-IV, Kynurenine Aminotransferase 4.
Greater than 90.0% as determined by SDS-PAGE.
Description
GOT2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 422 amino acids (30-430 aa) and having a molecular mass of 46.8kDa.
GOT2 is fused to a 21 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Transaminase A, KAT4, KATIV, KAT-4, KAT-IV, Kynurenine Aminotransferase 4.
Escherichia Coli.
MGSSHHHHHH SSGLVPRGSH MSSWWTHVEM GPPDPILGVT EAFKRDTNSK KMNLGVGAYR
DDNGKPYVLP SVRKAEAQIA AKNLDKEYLP IGGLAEFCKA SAELALGENN EVLKSGRFVT
VQTISGTGAL RVGASFLQRF FKFSRDVFLP KPSWGNHTPI FRDAGMQLQG YRYYDPKTCG
FDFSGALEDI SKIPEQSVLL LHACAHNPTG VDPRPEQWKE IASVVKKKNL FAFFDMAYQG
FASGDGDKDA WAVRHFIEQG INVCLCQSYA KNMGLYGERV GAFTVVCKDA EEAKRVESQL
KILIRPLYSN PPLNGARIAA TILTSPDLRK QWLQEVKGMA DRIISMRTQL VSNLKKEGSS
HNWQHITDQI GMFCFTGLKP EQVERLTKEF SVYMTKDGRI SVAGVTSGNV GYLAHAIHQV TK
Product Science Overview
Glutamic-Oxaloacetic Transaminase 2 (GOT2), also known as Aspartate Aminotransferase 2 (AST2), is a pyridoxal phosphate-dependent enzyme that plays a crucial role in amino acid metabolism and the urea and tricarboxylic acid cycles . This enzyme exists in both cytoplasmic and mitochondrial forms, with GOT2 being the mitochondrial variant .
GOT2 is a homodimeric enzyme, meaning it consists of two identical subunits. Each subunit contains several domains, including a large domain that binds pyridoxal phosphate, a small domain, an NH2-terminal arm, and a bridge formed by specific residues . The enzyme catalyzes the transamination of oxaloacetate to aspartate, a key reaction in the malate-aspartate shuttle, which is essential for transferring reducing equivalents across the mitochondrial membrane .
GOT2 is integral to the malate-aspartate shuttle, which facilitates the transfer of NADH from the cytosol to the mitochondria for energy production . This shuttle is vital for maintaining the NAD+/NADH balance within cells, which is crucial for various metabolic processes, including glycolysis and the electron transport chain .
Recombinant GOT2, particularly the mouse variant, is produced in E. coli and is used extensively in research. The recombinant protein is a single, non-glycosylated polypeptide chain containing 422 amino acids and has a molecular mass of approximately 46.8 kDa . It is often fused to a His-tag at the N-terminus to facilitate purification .
Recombinant GOT2 is used in various biochemical assays to study its role in amino acid metabolism and its involvement in diseases such as developmental and epileptic encephalopathy and pheochromocytoma . Additionally, GOT2’s role in the malate-aspartate shuttle makes it a target for research into metabolic disorders and potential therapeutic interventions .
