GMFB Antibody
Glia maturation factor beta, GMFB, GMF-B, GMF-beta, GMF.
Description
Product Specs
GMF-Beta stimulates the differentiation of both neurons and glial cells. It exhibits inhibitory effects on the proliferation of N-18 neuroblastoma and C6 glioma cell lines while promoting their phenotypic expression.
GMFB enhances the phenotypic expression of glia and neurons, thereby inhibiting the proliferation of their respective tumor cells in cell culture. While astrocytes produce and store GMF-b intracellularly, they do not secrete it into the culture medium. Cell-surface GMFb acts locally on target cells through direct contact. Produced by thymic epithelial cells, GMF-Beta plays a crucial role in T cell development, particularly favoring CD4+ T cells.
As a brain-specific protein belonging to the actin-binding proteins (ADF) family, GMF-beta is implicated in the differentiation, maintenance, and regeneration of the nervous system. It may also contribute to the progression of certain autoimmune diseases, potentially through its ability to induce the production and secretion of pro-inflammatory cytokines.
The solution contains 1mg/ml of GMFB in a buffer of PBS at pH 7.4, supplemented with 10% Glycerol and 0.02% Sodium Azide.
The GMFB antibody has been validated using ELISA, Western blot, and ICC/IF techniques to confirm its specificity and reactivity. However, optimal working concentrations may vary depending on the specific application and should be determined empirically through titration.
Glia maturation factor beta, GMFB, GMF-B, GMF-beta, GMF.
GMFB antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT44D8AT.
Anti-human GMFB mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GMFB amino acids 1-142 purified from E. coli.
Mouse IgG2a heavy chain and Kappa light chain.
Product Science Overview
GMFB is known to:
- Promote differentiation of brain cells: It stimulates the maturation of glial cells, which support and protect neurons.
- Stimulate neural regeneration: GMFB aids in the repair and regeneration of neural tissues.
- Inhibit tumor cell proliferation: It has been shown to inhibit the growth of certain tumor cells, making it a potential target for cancer therapy .
GMFB has been associated with various diseases and conditions:
- Glial Tumors: Abnormal expression of GMFB has been linked to the development of glial tumors .
- Diabetic Osteoporosis: GMFB deficiency has been found to protect against diabetic osteoporosis by suppressing osteoclast hyperactivity .
- Neuroinflammation: GMFB is a pro-inflammatory protein that contributes to neuroinflammation following brain injuries such as traumatic brain injury (TBI) .
Mouse anti-human GMFB antibodies are monoclonal antibodies produced in mice that specifically target human GMFB. These antibodies are used in various research applications, including:
- Immunohistochemistry: To detect GMFB expression in tissue samples.
- Western Blotting: To analyze GMFB protein levels in different samples.
- Functional Studies: To investigate the role of GMFB in various cellular processes and disease models.
Research on GMFB continues to uncover its potential therapeutic applications:
- Neurodegenerative Diseases: Targeting GMFB may help in treating neurodegenerative diseases by reducing neuroinflammation and promoting neural regeneration .
- Cancer Therapy: Inhibiting GMFB could be a strategy to prevent the proliferation of certain tumor cells .
- Osteoporosis Treatment: GMFB inhibitors have shown promise in treating osteoporosis related to type 1 diabetes by reducing osteoclast activity .
In conclusion, GMFB is a multifunctional protein with significant roles in glial cell maturation, neural regeneration, and disease modulation. Mouse anti-human GMFB antibodies are valuable tools in research, helping to elucidate the functions and therapeutic potential of GMFB.
