GMFB Mouse
Glia Maturation Factor Beta Mouse Recombinant
Cat. No.
BT9052
Source
Escherichia Coli.
Synonyms
Glia maturation factor beta, GMFB, GMF-B, GMF-beta, GMF, C79176, AI851627, D14Ertd630e, 3110001H22Rik, 3110001O16Rik.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Glia Maturation Factor-Beta (GMF-Beta) Mouse Recombinant produced in E.Coli is a signle, non-glycosylated, polypeptide chain containing 141 amino acids and having a total molecular mass of 16.6kDa.
GMF-Beta, Mouse Recombinant is purified by proprietary chromatographic techniques.
GMF-Beta, Mouse Recombinant is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Glia Maturation Factor-Beta (GMFB), a member of the GMF subfamily within the ADF family of actin-binding proteins, plays a critical role in the nervous system. Phosphorylated upon phorbol ester stimulation, GMFB is essential for brain cell differentiation, neural regeneration, and tumor cell proliferation inhibition. Notably, its overexpression in astrocytes enhances Brain-Derived Neurotrophic Factor (BDNF) production. Exercise-induced upregulation of GMFB highlights the significance of BDNF in this process.
Description
Recombinant Mouse Glia Maturation Factor-Beta (GMF-Beta), produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 141 amino acids, resulting in a molecular weight of 16.6 kDa. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The lyophilized GMF-beta protein is prepared in a 0.2µm filtered concentrated solution using PBS at a pH of 7.4.
Solubility
For reconstitution, it is advised to dissolve the lyophilized GMFB in sterile 18MΩ-cm H2O to a minimum concentration of 100µg/ml. This solution can be further diluted with other aqueous solutions as needed.
Stability
While lyophilized GMF-B remains stable at room temperature for up to 3 weeks, storage in a desiccated state below -18°C is recommended. After reconstitution, GMF-beta should be kept at 4°C for 2-7 days. For extended storage, freezing below -18°C is advisable. To ensure optimal stability during long-term storage, adding a carrier protein like HSA or BSA (0.1%) is recommended. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 97.0% is confirmed through the following analyses:
(a) RP-HPLC
(b) SDS-PAGE
Synonyms
Glia maturation factor beta, GMFB, GMF-B, GMF-beta, GMF, C79176, AI851627, D14Ertd630e, 3110001H22Rik, 3110001O16Rik.
Source
Escherichia Coli.
Amino Acid Sequence
SESLVVCDVA EDLVEKLRKF RFRKETHNAA IIMKIDKDER LVVLDEELEG
VSPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPVGCKPEQQ
MMYAGSKNKL VQTAELTKVF EIRNTEDLTE EWLREKLGFF H.
VSPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPVGCKPEQQ
MMYAGSKNKL VQTAELTKVF EIRNTEDLTE EWLREKLGFF H.
Product Science Overview
Introduction
Glia Maturation Factor Beta (GMF-β) is a protein that plays a crucial role in the development and maintenance of the nervous system. It belongs to the actin-binding proteins ADF family, specifically the GMF subfamily . This protein is involved in various cellular processes, including differentiation, maintenance, and regeneration of glial cells and neurons .
Structure and Properties
GMF-β contains an ADF-H (actin depolymerization factor homology) domain, which is essential for its function . The structures of mouse GMF-β, solved by both crystallography and NMR, reveal similarities and critical differences with ADF-H domains . The molecular weight of recombinant mouse GMF-β is approximately 16.6 kDa . It is typically produced in E. coli and is available as a sterile filtered white lyophilized (freeze-dried) powder .
Biological Functions
GMF-β is involved in several key biological processes:
- Nervous System Development: GMF-β is crucial for the differentiation and maintenance of glial cells and neurons .
- Immune Function: It plays a role in immune responses and has been implicated in angiogenesis .
- Tumor Cell Proliferation: GMF-β has been shown to inhibit the proliferation of tumor cells .
Research and Applications
Research on GMF-β has revealed its potential therapeutic applications:
- Diabetic Osteoporosis: GMF-β deficiency has been shown to protect against diabetic osteoporosis by suppressing osteoclast hyperactivity . This suggests that GMF-β inhibitors could be a potential therapeutic strategy for treating osteoporosis in diabetic patients.
- Neurodegenerative Diseases: Given its role in nervous system development and maintenance, GMF-β is being studied for its potential in treating neurodegenerative diseases .
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