GMF B Rat
Glia Maturation Factor Beta Rat Recombinant
Cat. No.
BT8836
Source
Escherichia Coli.
Synonyms
Glia maturation factor beta, GMFB, GMF-B, GMF-beta, GMF, C79176, AI851627, D14Ertd630e, 3110001H22Rik, 3110001O16Rik.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Glia Maturation Factor-Beta (GMF-Beta) Rat Recombinant produced in E.Coli is a signle, non-glycosylated, polypeptide chain containing 141 amino acids and having a total molecular mass of 16.6kDa.
GMF-Beta is purified by proprietary chromatographic techniques.
GMF-Beta is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Glia Maturation Factor-Beta (GMF-Beta), a member of the GMF subfamily within the ADF family of actin-binding proteins, plays a critical role in the nervous system. Its phosphorylation is triggered by phorbol ester stimulation. GMF-Beta is essential for brain cell differentiation, promotes neural regeneration, and inhibits tumor cell proliferation. Overexpression of GMF-Beta in astrocytes leads to increased production of Brain-Derived Neurotrophic Factor (BDNF). Exercise also elevates GMF-Beta expression, highlighting the significance of BDNF in exercise-induced BDNF production.
Description
Recombinant Rat Glia Maturation Factor-Beta (GMF-Beta), expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 141 amino acids with a molecular weight of 16.6 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 0.2 µm filtered solution at a concentration of 1.0 mg/ml in 1X PBS, pH 7.4.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized GMF-Beta in sterile 18 MΩ-cm H₂O at a minimum concentration of 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized GMF-Beta remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. After reconstitution, GMF-Beta should be stored at 4°C for 2-7 days. For future use, it should be stored below -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0% as determined by:
(a) RP-HPLC analysis.
(b) SDS-PAGE analysis.
Synonyms
Glia maturation factor beta, GMFB, GMF-B, GMF-beta, GMF, C79176, AI851627, D14Ertd630e, 3110001H22Rik, 3110001O16Rik.
Source
Escherichia Coli.
Amino Acid Sequence
SESLVVCDVA EDLVEKLRKF RFRKETHNAA IIMKIDKDKR LVVLDEELEG VSPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPLGCKPEQQ MMYAGSKNKL VQTAELTKVF EIRNTEDLTE EWLREKLGFF H.
Product Science Overview
Introduction
Glia Maturation Factor Beta (GMFB) is a protein that plays a crucial role in the development and differentiation of glial cells and neurons. It belongs to the actin-binding proteins ADF family, specifically the GMF subfamily . GMFB is involved in various biological processes, including neural regeneration, immune function, and angiogenesis .
Structure and Properties
GMFB contains an ADF-H domain, which is a characteristic feature of actin-binding proteins . The recombinant rat GMFB is typically produced in E. coli and has a molecular weight of approximately 16.6 kDa . The protein is usually provided as a sterile, lyophilized powder with a purity greater than 98% as determined by SDS-PAGE and HPLC analyses .
Biological Functions
GMFB is known to influence several critical biological functions:
- Neural Differentiation and Regeneration: GMFB promotes the differentiation of brain cells and stimulates neural regeneration .
- Inhibition of Tumor Cell Proliferation: It has been shown to inhibit the proliferation of tumor cells, making it a potential target for cancer research .
- Osteoclast Activity: Recent studies have indicated that GMFB plays a role in osteoclast activation, particularly in the context of diabetic osteoporosis . GMFB deficiency has been shown to ameliorate the phenotype of type 1 diabetes-induced osteoporosis by inhibiting osteoclast hyperactivity .
Research and Applications
Research on GMFB has revealed its potential therapeutic applications:
- Diabetic Osteoporosis: GMFB deficiency has been found to protect against diabetic osteoporosis by suppressing osteoclast hyperactivity . This discovery opens new avenues for the treatment of osteoporosis secondary to type 1 diabetes.
- Cancer Research: Due to its ability to inhibit tumor cell proliferation, GMFB is being explored as a potential therapeutic target in cancer treatment .
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