GMFG Human

Glia Maturation Factor Gamma (GMF-Gamma) is a protein that plays a significant role in the development and function of the nervous system and immune system. This article delves into the background, structure, function, and applications of GMF-Gamma, particularly focusing on its human recombinant form.

GMF-Gamma is a member of the glia maturation factor family, which includes proteins involved in the differentiation and maturation of glial cells. The human recombinant form of GMF-Gamma is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain consisting of 142 amino acids. The molecular mass of this protein is approximately 16.8 kDa .

GMF-Gamma is primarily expressed in hematopoietic cells and is known to mediate the pluripotentiality and lineage commitment of human hematopoietic stem cells . It is a cytokine-responsive protein involved in erythropoietin (EPO)-induced and granulocyte colony-stimulating factor (G-CSF)-induced hematopoietic lineage development . Additionally, GMF-Gamma acts as a nerve growth factor, contributing to nervous system development, angiogenesis, and immune function .

In the immune system, GMF-Gamma regulates the migration and adherence of human T lymphocytes. It is a component of the pseudopodia of migrating T cells and is essential for cell polarization and the extension of pseudopodia in the direction of movement . Inhibition of GMF-Gamma expression in T cells results in reduced migration and increased adherence to extracellular matrix proteins, indicating its role in integrin-mediated adhesion .

The human recombinant form of GMF-Gamma is used in various research applications, particularly in studies related to hematopoiesis, neurobiology, and immunology. Its ability to influence cell migration and adhesion makes it a valuable tool for understanding the molecular mechanisms underlying these processes.