FKBP1B Human
FK506 Binding Protein 1B Human Recombinant
Cat. No.
BT20346
Source
Escherichia Coli.
Synonyms
Peptidyl-prolyl cis-trans isomerase FKBP1B, PPIase FKBP1B, 12.6 kDa FK506-binding protein, 12.6 kDa FKBP, FKBP-12.6, FK506-binding protein 1B, FKBP-1B, Immunophilin FKBP12.6, Rotamase, h-FKBP-12, FKBP1B, FKBP12.6, FKBP1L, FKBP9, OTK4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE analysis.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
FKBP1B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 130 amino acids (1-108) and having a molecular mass of 14.2kDa.
FKBP1B is fused to a 22 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
FKBP1B is fused to a 22 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Peptidyl-prolyl cis-trans isomerase FKBP1B, also known as FKBP1B, plays a crucial role in immunoregulation and fundamental cellular processes like protein folding and trafficking. This protein belongs to the immunophilin family and functions as a cis-trans prolyl isomerase. It exhibits binding affinity for the immunosuppressants FK506 and rapamycin. Notably, FKBP1B shares significant similarity with FK506-binding protein 1A. Regarding its physiological function, FKBP1B is believed to be involved in excitation-contraction coupling within cardiac muscle.
Description
Recombinant human FKBP1B, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein comprises 130 amino acids, spanning residues 1 to 108, with a molecular weight of 14.2 kDa. For purification purposes, FKBP1B is fused to a 22-amino acid His-tag at the N-terminus and subsequently purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterilized by filtration.
Formulation
The FKBP1B solution is provided at a concentration of 0.25 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. To further enhance long-term stability, the addition of a carrier protein, such as HSA or BSA, at a concentration of 0.1% is advised. It's important to avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the protein is determined to be greater than 90.0% based on SDS-PAGE analysis.
Biological Activity
The specific activity of the enzyme is measured to be greater than 300 nmoles/min/mg. Specific activity represents the amount of enzyme required to cleave 1 µmol of the substrate suc-AAFP-pNA per minute at a temperature of 25°C. The assay is performed in Tris-HCl buffer at pH 8.0, using chymotrypsin as a reference.
Synonyms
Peptidyl-prolyl cis-trans isomerase FKBP1B, PPIase FKBP1B, 12.6 kDa FK506-binding protein, 12.6 kDa FKBP, FKBP-12.6, FK506-binding protein 1B, FKBP-1B, Immunophilin FKBP12.6, Rotamase, h-FKBP-12, FKBP1B, FKBP12.6, FKBP1L, FKBP9, OTK4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH RSMGVEIETI SPGDGRTFPK KGQTCVVHYT GMLQNGKKFD SSRDRNKPFK FRIGKQEVIK GFEEGAAQMS LGQRAKLTCT PDVAYGATGH PGVIPPNATL IFDVELLNLE.
Product Science Overview
Introduction
FK506 Binding Protein 1B (FKBP1B), also known as FKBP12.6, is a member of the FK506-binding protein family. These proteins are known for their role in immunosuppression and protein folding. FKBP1B is a human recombinant protein that has been extensively studied for its biological and therapeutic significance.
Structure and Function
Role in Immunosuppression
FKBP1B binds to the immunosuppressant drug FK506 (tacrolimus), forming a complex that inhibits the phosphatase activity of calcineurin. This inhibition prevents the activation of T-lymphocytes, thereby suppressing the immune response. This mechanism is particularly important in preventing organ rejection in transplant patients .
Biological Significance
- Cardiac Function: FKBP1B is predominantly expressed in cardiac muscle and plays a critical role in regulating calcium release from the sarcoplasmic reticulum. This regulation is essential for proper cardiac muscle contraction and overall heart function .
- Neurological Function: FKBP1B has been implicated in neuronal function and has been studied for its potential role in neurodegenerative diseases. It is believed to interact with various proteins involved in neuronal signaling pathways .
Therapeutic Applications
The ability of FKBP1B to bind FK506 and inhibit calcineurin has made it a target for therapeutic interventions in autoimmune diseases and organ transplantation. Additionally, its role in cardiac and neurological functions opens avenues for research into treatments for heart diseases and neurodegenerative disorders .
Research and Development
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