FKBP3 Human

FK506 Binding Protein 3 Human Recombinant
Cat. No.
BT20524
Source
Escherichia Coli.
Synonyms
EC 5.2.1.8, FKBP-3, FKBP25, FKBP-25, Peptidyl-prolyl cis-trans isomerase FKBP3, PPIase FKBP3, FK506-binding protein 3, Rotamase, Immunophilin FKBP25, 25 kDa FK506-binding protein, 25 kDa FKBP, Rapamycin-selective 25 kDa immunophilin, FKBP3, PPIase.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

FKBP3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 224 amino acids (1-224 a.a.) and having a molecular mass of 25.1 kDa. FKBP3 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
FKBP3, a member of the immunosuppressant receptor family, plays a crucial role in inhibiting T-cell proliferation. It achieves this by halting two distinct cytoplasmic signal transmission pathways. Functioning as a protein folding facilitator, FKBP3 belongs to the immunophilin protein family, which is involved in immunoregulation and essential cellular processes such as protein folding and trafficking. This cis-trans prolyl isomerase enzyme exhibits binding affinity for immunosuppressants like FK506 and rapamycin, in addition to histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. Notably, its affinity for rapamycin surpasses that of FK506, highlighting its significance in rapamycin-mediated immunosuppression.
Description
Produced in E.Coli, FKBP3 is a single, non-glycosylated polypeptide chain consisting of 224 amino acids (amino acids 1-224). Its molecular weight is 25.1 kDa. The purification of FKBP3 is carried out using proprietary chromatographic techniques.
Physical Appearance
A colorless solution that has been sterilized by filtration.
Formulation
The FKBP3 protein solution is formulated with 20mM Tris-HCl at a pH of 8, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
SDS-PAGE analysis indicates a purity greater than 90.0%.
Biological Activity

The specific activity, determined by measuring the enzyme's ability to cleave 1 µmole of suc-AAFP-pNA per minute at 37°C in Tris-Hcl pH 8.0 using chymotrypsin as a reference, is greater than 900 nmoles/min/mg.

Synonyms
EC 5.2.1.8, FKBP-3, FKBP25, FKBP-25, Peptidyl-prolyl cis-trans isomerase FKBP3, PPIase FKBP3, FK506-binding protein 3, Rotamase, Immunophilin FKBP25, 25 kDa FK506-binding protein, 25 kDa FKBP, Rapamycin-selective 25 kDa immunophilin, FKBP3, PPIase.
Source
Escherichia Coli.
Amino Acid Sequence
MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID.

Product Science Overview

Structure and Function

FKBP3 is characterized by its peptidyl-prolyl cis-trans isomerase (PPIase) activity, which facilitates protein folding by catalyzing the isomerization of proline residues in polypeptides . This activity is crucial for the proper folding and function of many proteins within the cell.

In addition to its PPIase activity, FKBP3 has been found to play a role in various cellular processes, including:

  • Protein Folding: FKBP3 acts as a chaperone, assisting in the proper folding of newly synthesized proteins .
  • DNA Repair: FKBP3 has been shown to bind non-specifically to DNA and is involved in DNA repair mechanisms .
  • Signal Transduction: By forming complexes with other proteins, FKBP3 can influence various signaling pathways within the cell .
Role in Immunosuppression

One of the most notable functions of FKBP3 is its role in immunosuppression. When bound to FK506, FKBP3 forms a complex that inhibits the phosphatase activity of calcineurin . This inhibition prevents the activation of the nuclear factor of activated T-cells (NF-AT), thereby blocking T-cell activation and proliferation. This mechanism is particularly important in preventing organ rejection in transplant patients and in treating autoimmune disorders .

Research and Therapeutic Applications

FKBP3 and other members of the FKBP family have become subjects of considerable interest in various fields of research due to their involvement in numerous cellular and molecular pathways . Some key areas of research and therapeutic applications include:

  • Cancer Research: FKBP3 has been implicated in the regulation of cell growth and apoptosis, making it a potential target for cancer therapy .
  • Neurodegenerative Diseases: The role of FKBP3 in protein folding and stress response pathways suggests its potential involvement in neurodegenerative diseases such as Alzheimer’s and Parkinson’s .
  • Drug Development: Understanding the binding mechanisms of FK506 and rapamycin to FKBP3 can aid in the development of new immunosuppressive drugs and other therapeutic agents .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

FKBP14 Human
FK506 Binding Protein 14 Human Recombinant
Cat. No.
BT20056
Source
Escherichia Coli.
FKBP1A Human
FK506 Binding Protein 1A Human Recombinant
Cat. No.
BT20143
Source
Escherichia Coli.
FKBP1B Human
FK506 Binding Protein 1B Human Recombinant
Cat. No.
BT20346
Source
Escherichia Coli.
FKBPL Human
FK506 Binding Protein Like Human Recombinant
Cat. No.
BT20890
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.