FKBPL Human

FK506 Binding Protein Like Human Recombinant
Cat. No.
BT20890
Source
Escherichia Coli.
Synonyms
WISP39, DIR1, NG7, FK506-binding protein-like, WAF-1/CIP1 stabilizing protein 39, FKBPL.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FKBPL Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 357 amino acids (1-349 a.a.) and having a molecular mass of 39.2 kDa. The FKBPL is fused to an 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
FKBPL, a member of the immunophilin protein family, plays a crucial role in immunoregulation and essential cellular processes such as protein folding and trafficking. It is involved in induced radioresistance, cell cycle control, and cellular stress response. FKBPL interacts with proteins like Hsp90, glucocorticoid receptor, and dynamitin, participating in signaling pathways similar to other FKBPs.
Description
Recombinant Human FKBPL, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 357 amino acids (residues 1-349) with a molecular weight of 39.2 kDa. An 8 amino acid His-Tag is fused to the C-terminus of FKBPL, and the protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
FKBPL Human solution is supplied in 20mM Tris buffer (pH 8.0), containing 2mM DTT, 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Biological Activity
The specific activity, exceeding 210 nmoles/min/mg, is defined as the amount of enzyme required to cleave 1 μmole of suc-AAFP-pNA per minute at 25°C in Tris-HCl buffer (pH 8.0) using chymotrypsin as a substrate.
Synonyms
WISP39, DIR1, NG7, FK506-binding protein-like, WAF-1/CIP1 stabilizing protein 39, FKBPL.
Source
Escherichia Coli.
Amino Acid Sequence

METPPVNTIG EKDTSQPQQE WEKNLRENLD SVIQIRQQPR DPPTETLELE VSPDPASQIL EHTQGAEKLV AELEGDSHKS HGSTSQMPEA LQASDLWYCP DGSFVKKIVI RGHGLDKPKL GSCCRVLALG FPFGSGPPEG WTELTMGVGP WREETWGELI EKCLESMCQG EEAELQLPGH SGPPVRLTLA SFTQGRDSWE LETSEKEALA REERARGTEL FRAGNPEGAA RCYGRALRLL LTLPPPGPPE RTVLHANLAA CQLLLGQPQL AAQSCDRVLE REPGHLKALY RRGVAQAALG NLEKATADLK KVLAIDPKNR AAQEELGKVV IQGKNQDAGL AQGLRKMFGL EHHHHHH.

Product Science Overview

Introduction

FK506 Binding Proteins (FKBPs) are a family of proteins that have garnered significant interest due to their role in various cellular processes and their interaction with immunosuppressive drugs such as FK506 (tacrolimus) and rapamycin . These proteins are known for their peptidyl-prolyl cis-trans isomerase (PPIase) activity, which aids in protein folding and function .

Structure and Function

FKBPs are characterized by their ability to bind to FK506 and rapamycin, forming complexes that inhibit the phosphatase activity of calcineurin . This inhibition is crucial for the immunosuppressive effects of these drugs, particularly in preventing organ rejection post-transplantation . The FKBP family includes several members, each with distinct functions and tissue distributions .

FKBP12

One of the most studied members of this family is FKBP12. It binds to FK506 and rapamycin, inhibiting calcineurin and thus blocking T-cell activation . FKBP12 is also involved in the regulation of calcium channels, such as the ryanodine receptor, which plays a critical role in calcium signaling pathways .

FKBP51 and FKBP52

FKBP51 and FKBP52 are larger FKBPs that function as co-chaperones with heat shock protein 90 (Hsp90) in steroid receptor complexes . These proteins are involved in the regulation of steroid hormone receptors and have been implicated in various diseases, including cancer and psychiatric disorders .

FKBP14

FKBP14 is another member of the FKBP family that has been shown to interact with components of the Notch signaling pathway . This interaction is essential for the proper functioning of the pathway, which is crucial for cell differentiation and development .

Applications in Research and Medicine

The unique properties of FKBPs have made them valuable tools in biological research. For instance, FKBP12 can be used in chemically induced dimerization applications to manipulate protein localization and signaling pathways . Additionally, the immunosuppressive properties of FKBP-binding drugs have been harnessed in clinical settings to prevent organ rejection and treat autoimmune diseases .

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