FKBP4 Human

FK506 Binding Protein 4 Human Recombinant
Cat. No.
BT20597
Source
Escherichia Coli.
Synonyms
HBI, p52, Hsp56, FKBP52, FKBP59, PPIase, FKBP4, FK506-binding protein 4, Peptidyl-prolyl cis-trans isomerase, HSP-binding immunophilin, FKBP52 protein, 52 kDa FK506-binding protein, p59 protein.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FKBP4 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 479 amino acids (1-459 a.a.) and having a molecular mass of 53.9 kDa.
FKBP4 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
FKBP4, a member of the immunophilin protein family, plays a crucial role in immunoregulation and essential cellular processes like protein folding and trafficking. This cis-trans prolyl isomerase interacts with immunosuppressants such as FK506 and rapamycin. Despite its structural and functional similarities to FKBP1A, FKBP4 does not exhibit immunosuppressant activity when bound to FK506. It interacts with phytanoyl-CoA alpha-hydroxylase and associates with HSP90 and HSP70, participating in the intracellular trafficking of hetero-oligomeric forms of steroid hormone receptors. Notably, FKBP4 exhibits strong association with adeno-associated virus type 2 vectors (AAV), significantly enhancing AAV-mediated transgene expression in human cell lines, making it crucial for optimizing AAV vectors in human gene therapy.
Description
Produced in E. coli, this FKBP4 protein is a single, non-glycosylated polypeptide chain comprising 479 amino acids (1-459 a.a.) with a molecular weight of 53.9 kDa. A 20 amino acid His Tag is fused to the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
This FKBP4 protein solution is supplied in a buffer containing 20mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of this protein is greater than 90.0% as determined by SDS-PAGE analysis.
Biological Activity
The specific activity, a measure of the enzyme's ability to cleave suc-AAFP-pNA, is greater than 300 nmoles/min/mg. This is determined at 25°C in Tris-Hcl pH 8.0 using chymotrypsin as a reference.
Synonyms
HBI, p52, Hsp56, FKBP52, FKBP59, PPIase, FKBP4, FK506-binding protein 4, Peptidyl-prolyl cis-trans isomerase, HSP-binding immunophilin, FKBP52 protein, 52 kDa FK506-binding protein, p59 protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFDLGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKDKLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVYFKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVLQLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA.

Product Science Overview

Introduction

FK506 Binding Protein 4 (FKBP4), also known as FKBP52, is a member of the immunophilin protein family. This family of proteins is involved in immunoregulation and essential cellular processes such as protein folding and trafficking . FKBP4 is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 (tacrolimus) and rapamycin .

Structure and Function

FKBP4 is characterized by its ability to catalyze the cis-trans isomerization of proline residues in proteins, which is crucial for proper protein folding. This protein contains multiple domains, including the FK506-binding domain and the tetratricopeptide repeat (TPR) domain. The TPR domain is involved in protein-protein interactions and is essential for the protein’s role as a co-chaperone in the heat shock protein 90 (Hsp90) complex .

Role in Immunoregulation

FKBP4, along with other FKBPs, plays a significant role in immunoregulation by binding to immunosuppressive drugs. The FKBP-FK506 complex inhibits the phosphatase activity of calcineurin, thereby blocking the activation of T-cells and preventing immune responses . This mechanism is particularly important in preventing organ rejection in transplant patients and treating autoimmune diseases .

Cellular Processes

Beyond its role in immunoregulation, FKBP4 is involved in various cellular processes. It acts as a co-chaperone with Hsp90, assisting in the proper folding and stabilization of steroid hormone receptors and other client proteins . FKBP4 also plays a role in the trafficking of proteins within the cell, ensuring that they reach their correct destinations .

Research and Applications

The recombinant form of FKBP4, expressed in Escherichia coli, is widely used in research to study its structure and function. This recombinant protein is valuable for investigating the molecular mechanisms underlying its interactions with immunosuppressive drugs and its role in cellular processes . Additionally, FKBP4 is a potential target for therapeutic interventions in diseases related to protein misfolding and trafficking .

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