ENO2 Mouse
Enolase-2 Mouse Recombinant
Cat. No.
BT16660
Source
Escherichia Coli.
Synonyms
AI837106, D6Ertd375e, Eno-2, NSE, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ENO2 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 457 amino acids (1-434) and having a molecular mass of 49.7kDa.ENO2 is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
Product Specs
Introduction
Neuron-specific enolase (NSE), also known as Enolase-2, is a glycolytic isoenzyme primarily found in central and peripheral neurons and neuroendocrine cells. Its presence in the cerebrospinal fluid (CSF) can indicate neural tissue injury. NSE is released into the bloodstream by neoplasms originating from neural or neuroendocrine tissues. As a result, it serves as a valuable biomarker for detecting and monitoring certain tumors, including neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. ENO2 is one of three enolase isoenzymes found in mammals. The ENO2 isoenzyme is specifically found in mature neurons and cells of neuronal origin. During development in rats and primates, a transition from alpha enolase to gamma enolase occurs in neural tissue.
Description
Recombinant Mouse ENO2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 457 amino acids (with amino acids 1-434 representing the ENO2 protein) and has a molecular weight of 49.7 kDa. The ENO2 protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ENO2 solution is provided at a concentration of 1 mg/ml and is formulated in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), store the vial at 4°C. For extended storage, freeze the product at -20°C. To ensure long-term stability during frozen storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
Purity is determined to be greater than 95% using SDS-PAGE analysis.
Biological Activity
The specific activity of the recombinant ENO2 is measured to be greater than 10,000 pmol/min/µg. This value is determined by assessing the decrease in absorbance at 340 nm due to the conversion of NADH to NAD at pH 6.5 and 37°C.
Synonyms
AI837106, D6Ertd375e, Eno-2, NSE, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSIEKIW AREILDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDGDKQRY LGKGVLKAVD HINSRIAPAL ISSGISVVEQ EKLDNLMLEL DGTENKSKFG ANAILGVSLA VCKAGAAERD LPLYRHIAQL AGNSDLILPV PAFNVINGGS HAGNKLAMQE FMILPVGAES FRDAMRLGAE VYHTLKGVIK DKYGKDATNV GDEGGFAPNI LENSEALELV KEAIDKAGYT EKMVIGMDVA ASEFYRDGKY DLDFKSPADP SRYITGDQLG ALYQDFVRNY PVVSIEDPFD QDDWAAWSKF TANVGIQIVG DDLTVTNPKR IERAVEEKAC NCLLLKVNQI GSVTEAIQAC KLAQENGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQLMRIEEEL GDEARFAGHN FRNPSVL.
Product Science Overview
Structure and Function
Enolase-2 is a dimeric enzyme composed of two identical subunits. Each subunit has a molecular weight of approximately 47 kDa . The enzyme is highly expressed in neuronal tissues and neuroendocrine cells, making it a valuable marker for neuronal differentiation and neuroendocrine tumors .
The primary function of enolase-2 is to facilitate the glycolytic pathway, which is essential for energy production in cells. However, enolase-2 also exhibits “moonlighting” functions, meaning it can perform multiple roles unrelated to its glycolytic activity. For instance, enolase-2 has been implicated in processes such as cell growth, survival, and differentiation .
Recombinant Enolase-2
Recombinant enolase-2 refers to the enzyme produced through recombinant DNA technology. This involves inserting the gene encoding enolase-2 into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. Recombinant enolase-2 is used in various research applications, including studies on neuronal function, cancer research, and as a diagnostic marker for neuroendocrine tumors .
Applications in Research
- Neuroscience Research: Enolase-2 is widely used as a marker for neuronal cells. Its high expression in neurons makes it a valuable tool for studying neuronal differentiation and neurodegenerative diseases.
- Cancer Research: Enolase-2 is a well-known marker for neuroendocrine tumors. Elevated levels of enolase-2 are often observed in patients with neuroendocrine tumors, making it a useful diagnostic and prognostic marker.
- Metabolic Studies: Given its role in glycolysis, enolase-2 is also studied in the context of cellular metabolism and energy production.
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