ENO2 Antibody
Enolase-2, Mouse Anti Human
Cat. No.
BT16783
Source
Synonyms
Gamma-enolase, EC 4.2.1.11, 2-phospho-D-glycerate hydro-lyase, Neural enolase, Neuron-specific enolase, NSE, Enolase 2, ENO2.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Neuron-specific enolase, also known as NSE, is a glycolytic isoenzyme found in central and peripheral neurons and neuroendocrine cells. Enolase-2 is released into the cerebrospinal fluid (CSF) following neural tissue injury. Neoplasms originating from neural or neuroendocrine tissue release Enolase-2 into the bloodstream. Enolase-2 serves as a valuable biomarker and has been detected in patients diagnosed with specific tumors, including neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. ENO2 is one of three enolase isoenzymes found in mammals. The ENO2 isoenzyme is present in mature neurons and cells of neuronal origin. A transition from alpha enolase to gamma enolase takes place in neural tissue during the developmental stages of rats and primates.
Physical Appearance
Sterile, colorless solution.
Formulation
1 mg/ml in phosphate-buffered saline (PBS), pH 7.4, containing 10% glycerol and 0.02% sodium azide.
Storage Procedures
For storage up to 1 month, maintain at 4°C. For extended storage periods, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
This antibody has undergone testing by ELISA and Western blot analysis to ensure its specificity and reactivity. However, as applications may vary, it is recommended to titrate the reagent for each specific experiment to achieve optimal results. The suggested dilution range for Western blot analysis is 1:500 to 1:5000, with an initial starting dilution of 1:1000.
Synonyms
Gamma-enolase, EC 4.2.1.11, 2-phospho-D-glycerate hydro-lyase, Neural enolase, Neuron-specific enolase, NSE, Enolase 2, ENO2.
Purification Method
ENO2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT17D10A
Immunogen
Anti-human ENO2 mAb, clone PAT17D10A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ENO2 protein 1-434 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and Kappa light chain.
Product Science Overview
Structure and Function
Enolase-2 is one of the three isoforms of enolase, the others being enolase-1 (α-enolase) and enolase-3 (β-enolase). The enzyme exists as a homodimer or heterodimer, with the γγ homodimer being specific to neurons . The enzymatic site spans most of the length of the molecule, and it is highly conserved across species .
Clinical Significance
Mouse Anti Human Enolase-2 Antibody
The Mouse Anti Human Enolase-2 Antibody is a monoclonal antibody that specifically targets the human Enolase-2 protein. This antibody is produced by immunizing mice with a synthetic peptide corresponding to the sequence of human Enolase-2 . It is widely used in research for detecting Enolase-2 in various applications, including Western blotting, immunohistochemistry, and flow cytometry .
Applications
Species Reactivity
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