ENO2 Human, His
Single band on Western Blot.
Description
The NSE is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The recombinant human Enolase-2 protein is typically produced in E. coli and includes an N-terminal His-tag for purification purposes. The protein sequence ranges from Met1 to Leu434, with an additional N-terminal Met and a 6-His tag . The predicted molecular mass of this recombinant protein is approximately 48 kDa, although it may appear as 46 kDa under reducing conditions in SDS-PAGE .
Enolase-2 is highly active in converting phosphoglyceric acid to phosphoenolpyruvate, with a specific activity greater than 6,000 pmol/min/μg under the described conditions . This enzyme is crucial for energy production in neurons and is often used as a marker for neuronal damage and neuroendocrine tumors .
Recombinant Enolase-2 is widely used in research for various applications, including:
- Biomarker Studies: NSE is a well-known biomarker for neuronal damage and certain types of cancer, such as neuroblastoma and small cell lung cancer .
- Enzyme Activity Assays: It is used to study glycolytic enzyme activity and metabolic pathways in neurons .
- Therapeutic Research: Studies have shown that silencing ENO2, in combination with 2-deoxyglucose (2-DG), can inhibit leukemia cell survival, making it a potential target for cancer therapy .
The recombinant Enolase-2 protein is supplied as a 0.2 μm filtered solution in MES, NaCl, KCl, and MgSO₄. It should be stored at -20 to -70°C to maintain stability and avoid repeated freeze-thaw cycles . Under sterile conditions, it remains stable for up to 6 months from the date of receipt and 3 months after opening .
