EFNB3 Human

Ephrin-B3 is an approximately 50 kDa protein that plays a crucial role in the development and maintenance of the nervous system . It is a transmembrane protein with an intracellular tail containing highly conserved tyrosine residues and a PDZ-binding motif at the C-terminus . Ephrin-B3 interacts with EphB receptors, particularly EphB3, to mediate cell-cell communication and signaling pathways essential for various biological processes .

Ephrin-B3 is prominently expressed in the brain and is involved in brain development and maintenance . It is particularly important in the patterning of the nervous system, as evidenced by its expression at the dorsal and ventral midline of the neural tube in mouse embryos . Ephrin-B3’s interaction with EphB receptors influences axon guidance, cell migration, and the formation of neural circuits .

Recombinant human Ephrin-B3 is produced using a mouse myeloma cell line, NS0-derived human Ephrin-B3 protein . The recombinant protein is typically purified to a high degree of purity (>95%) and is used in various research applications, including functional assays and binding studies . It is available in both carrier-free and carrier-protein formulations, depending on the intended use .

Recombinant human Ephrin-B3 is used in research to study its binding ability and interactions with EphB receptors . It is also utilized in assays to investigate its role in cell signaling, development, and disease processes. The recombinant protein’s high purity and specific activity make it a valuable tool for researchers exploring the molecular mechanisms underlying ephrin-Eph receptor interactions .

Ephrin-B3’s role in the nervous system and its interactions with EphB receptors highlight its importance in developmental biology and neurobiology. Understanding the functions and mechanisms of Ephrin-B3 can provide insights into various neurological disorders and potential therapeutic targets.