EFNB3 Human, Sf9
Ephrin- B3 Human Recombinant, Sf9
Cat. No.
BT3311
Source
Sf9, Baculovirus cells.
Synonyms
Ephrin B3, EPH-Related Receptor Transmembrane Ligand ELK-L3, Ephrin-B3, EPLG8, LERK8, Eph-Related Receptor Tyrosine Kinase Ligand 8, EPH-Related Receptor Tyrosine Kinase Ligand 8, LERK-8, EFL6, Ephrin-B3, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8, LERK-8.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
EFNB3 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 208 amino acids (28-226a.a.) and
having a molecular mass of 23.0kDa. (Molecular size on SDS-PAGE will appear at
approximately 28-40kDa).
EFNB3 is expressed with a 6
amino acid His tag at C-Terminus and purified by proprietary chromatographic
techniques.
Product Specs
Introduction
Ephrin-B3 (EFNB3), a member of the ephrin family, plays a crucial role in brain development and maintenance. This protein interacts with and triggers the collapse of commissural axons/growth cones in vitro. By binding loosely to Eph receptors on adjacent cells, EFNB3 facilitates contact-dependent bidirectional signaling between neighboring cells. The EPH and EPH-related receptors constitute the largest subfamily of receptor protein-tyrosine kinases and are involved in mediating developmental processes, primarily in the nervous system.
Description
Produced in Sf9 Baculovirus cells, EFNB3 is a single, glycosylated polypeptide chain with a molecular weight of 23.0 kDa (208 amino acids, 28-226a.a.). On SDS-PAGE, its molecular size appears approximately between 28-40 kDa. The protein is expressed with a 6-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The EFNB3 protein solution is supplied at a concentration of 0.5 mg/ml in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
Ephrin B3, EPH-Related Receptor Transmembrane Ligand ELK-L3, Ephrin-B3, EPLG8, LERK8, Eph-Related Receptor Tyrosine Kinase Ligand 8, EPH-Related Receptor Tyrosine Kinase Ligand 8, LERK-8, EFL6, Ephrin-B3, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8, LERK-8.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPLSLEPVY
WNSANKRFQA EGGYVLYPQI GDRLDLLCPR ARPPGPHSSP NYEFYKLYLV GGAQGRRCEA PPAPNLLLTC
DRPDLDLRFT IKFQEYSPNL WGHEFRSHHD YYIIATSDGT REGLESLQGG VCLTRGMKVL LRVGQSPRGG
AVPRKPVSEM PMERDRGAAH SLEPGKENLP
GDPTSNATSR GAEGPLPPPS MPHHHHHH.
Product Science Overview
Structure and Expression
Recombinant human Ephrin-B3 is typically produced in baculovirus-infected Sf9 insect cells. The protein consists of amino acid residues 1-224, which correspond to the extracellular domain of human Ephrin-B3 . This domain is fused to the Fc portion of human IgG1, which is 6× histidine-tagged at the carboxyl terminus . The chimeric protein is expressed in an insect cell line, ensuring high purity and biological activity.
Biological Functions
Ephrin-B3 is involved in several critical developmental processes:
- Axon Guidance: Ephrin-B3, along with other ephrin-B family members, regulates the formation of major interhemispheric connections between the temporal lobes of the cerebral cortex. This includes the corpus callosum and the anterior commissure .
- Angiogenesis: Ephrin-B3 plays a role in the development of blood vessels by regulating cell migration and positioning .
- Cell Migration and Adhesion: The EFNB2/EPHB3 complex, which includes Ephrin-B3, regulates the migration and adhesion of cells involved in various developmental processes, such as palate development and thymic epithelium development .
Applications
Recombinant human Ephrin-B3 is used in various research applications, including:
- Functional ELISA: The binding ability of Ephrin-B3 can be measured using functional ELISA assays .
- SDS-PAGE: The protein’s purity and molecular weight can be analyzed using SDS-PAGE under reducing conditions .
- Cell Culture: Ephrin-B3 is used in cell culture studies to investigate its role in cell signaling and development .
Storage and Stability
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